阿托伐他汀对脑出血大鼠TNF-α和IL-10的影响

目的 观察不同剂量的阿托伐他汀对大鼠脑出血模型肿瘤坏死因子(TNF-α)、白细胞介素-10(IL-10)浓度的影响,探讨其神经保护作用的可能机制.方法 胶原酶诱发脑出血模型,ELISA检测血清、脑匀浆TNF-α、IL-10 动态变化;HE染色计数激活的小胶质细胞.结果 (1)与假手术组比较脑出血TNF-α、IL-10 的水平及激活的小胶质细胞增加(均P＜0.05).(2)与脑出血组相比阿托伐他汀干预组能减少激活的小胶质细胞(P＜0.05),能显著降低TNF-α水平(P＜0.05),能显著增加IL-10水平(P＜0.05),且在实验选取干预剂量中,随干预剂量的增大,调节作用有增强趋势.结论 上调IL-10下调TNF-α可能是阿托伐他汀减轻脑出血后急性炎性反应,阻止继发性脑损害,发挥神经保护作用的机制之一.

Influence of atorvastatin to TNF-α and IL-10 in intracerebral hemorrhage rats

Objective To observe the neuroprotective effect of atorvastatin and its influence to tumor necrosis factor-alpha(TNF-α) and intedeukin-10(IL-10)in intraeerebral hemorrhage(ICH)rats,and to explore the possible mechanism of its neuroproteetive effect.Methods ICH WaS induced by stereotaxic infusion of collagenase into the right baSal ganglia,the expressions of TNF-α,IL-10 in the serum and brain tissue were analyzed by enzyme linked immunosorbent assay(ELISA),HE staining was used to evaluate the number of activated microglia.Results (1)The numbers of activated mieroglia,TNF-α and IL-10 were have significant differences between group A and group B(all P＜0.05).(2)In comparision with group B,numbers of activated mieroglia in group C,D and E were significantly redueed(P＜0.05).Atorvastatin cause a dose-dependent regulation,which decreased the level of TNF-α and inereased the level of IL-10 significantly(all P＜0.05).Conclusion The down-regulation of TNF-α and up-regulation of IL-10 might partly attribute to the neuropmtection of atovastatin.