促酰化蛋白水平与儿童肥胖的关系

目的：儿童肥胖不仅是体内脂肪过度积蓄，它常延伸至成人，导致心血管疾病、糖尿病发病增高。促酰化蛋白(ASP)与脂肪代谢密切相关，据此该文测定儿童ASP及血脂水平，并分析与肥胖的相关性。 方法：将该院门诊就诊的单纯性肥胖儿童分为肥胖组(n=30)；正常体检儿童分为对照组(n=30)，分别测定ASP及血脂水平。ASP检测采用ELISA方法检测血浆ASP水平，试剂盒由加拿大麦吉尔大学医学中心皇家维多利亚医院心血管研究室提供，血脂检测采用免疫比浊法在全自动生化分析仪上检测。 结果：肥胖组血浆ASP、胆固醇、甘油三酯、载脂蛋白B依次为 73.87 ± 24.04 g/L ， 5.71 ±0.61 mmol/L， 1.77±0.30 mmol/L，0.98±0.20 mmol/L；对照组为 39.47±13.68 g/L，4.29±0.49 mmol/L，1.02±0.25 mmol/L，0.85±0.11 mmol/L。两组比较其差异有显著性意义(P<0.01或P<0.05)；ASP与体重指数(BMI)、血浆甘油三酯、胆固醇呈正相关(相关系数分别为r=0.43，0.48，0.68，分别P<0.05，P<0.05，P<0.01)；肥胖组中有肥胖家族史患儿血浆ASP水平比无家族史患儿增高更明显 103.4±10.57 g/L vs71.15±24.9 g/L，差异有显著性(P<0.01)。 结论：血浆ASP水平可作为脂质代谢检测的一种新指标，对判断儿童肥胖发展趋势和肥胖儿童未来发生心血管疾病危险度作出初步估价。

Relationship between plasma acylation stimulating protein and obesity in children

OBJECTIVE: To study the correlation of plasma acylation-stimulating protein (ASP) with body mass index (BMI) and blood lipid in children with obesity. METHODS: Thirty children with simple obesity and 30 healthy children were involved in this study. Plasma ASP levels were determined using ELISA. Blood lipid levels were detected with fully automatic biochemical analyser by turbidimetry. RESULTS: The levels of plasma ASP ( 73.87± 24.04 g/L vs 39.47± 13.68 g/L), cholesterol ( 5.71± 0.61 mmol/L vs 4.29± 0.49 mmol/L), triglyceride ( 1.77± 0.30 mmol/L vs 1.02± 0.25 mmol/L) and apoprotein B ( 0.98± 0.20 mmol/L vs 0.85± 0.11 mmol/L) increased significantly in obese children compared to those in the healthy children (P< 0.01 or P< 0.05). ASP was positively correlated to BMI, triglyceride and cholesterol levels (r= 0.43, P< 0.05; r= 0.48, P< 0.05; r= 0.68, P< 0.01, respectively). The ASP levels were significantly higher in the obese children with family history of obesity than those in the obese children without ( 103.4± 10.57 g/L vs 71.15± 24.9 g/L, P< 0.01). CONCLUSIONS: Plasma ASP may be used as a marker for evaluating fat metabolism in children with obesity. It might be valuable in predicting the possibility of developing cardiovascular diseases in their later lives.