醋酸棉酚长期给药的进一步观察

给成年雄性大鼠每日服醋酸棉酚10 mg/kg,每周服6天。服6个月后进行病理和组织化学观察。结果说明给醋酸棉酚大鼠各主要脏器(心、肝、脾、肺、肾、肾上腺)的形态都没有明显变化。组织化学观察说明,给棉酚大鼠的肝脏油红“O”染色、G-6-P酶、ATP酶、ALP酶、ACP酶、糖原、RNA和DNA肾脏G-6-PDH、油红“O”染色、ATP酶、ACP酶、ALP酶;肾上腺3β-甾体脱氢酶、油红“O”染色、苏丹黑染色等与对照组比较都没有明显差别。

FURTHER OBSERVATIONS ON THE EFFECT OF PROLONGED ADMINISTRATION OF GOSSYPOL ACETIC ACID TO RATS

Male rats were given gossypol acetic acid by mouth at a daily dosage of 10 mg/kg six days a week for six months. At the end of this period the main viscera were removed for pathological examinations. The chemical substances and enzymes in the adrenal glands, kidneys and livers were demonstrated by histochemical methods.Results showed that after six month treatment with gossypol acetic acid, no pathological changes were found under the light microscope in the heart, liver, spleen, lung, kidney, intestine, lymph node, bone marrow and blood picture.The 3 β-hydroxysteroid-dehydrogenase in the zona glomerulosa of the adrenal gland of the treated group was of slightly higher intensity which might indicate slightly higher enzyme activity. Oil red "O" and Sudan Black B stained specimens showed either slight increase or slight decrease in the number of fat droplets in the zona glomerulosa of the gossypol treated as compared with the control animals. No changein the renal G-6-PDH and renal ATPase activities was noted in the treated animals. The acid phosphatase activity of the proximal and distal tubules of the treated animals seemed to be slightly increased and the alkaline phosphatase activity of the proximal tubules showed very little changes. No variation from controls was observed in the ATPase, alkaline phosphatase and acid phosphatase activities of the liver. Liver glycogen, G-6-Pase and RNA did not differ from that of controls besides that there are some changes in a few cells. However, the G-6-PDH activity of the liver seemed to be slightly increased in gossypol treated rats.Liver glycogen contents as determined by the method of Van der Vies showed no significant difference between the control and gossypol treated animals.