内皮素和一氧化氮与新生儿缺氧缺血性脑病的研究

目的 探讨血浆与脑脊液中内皮素，一氧化氮在新生儿缺氧缺血性脑病中的作用及其相互关系。方法 对３５例ＨＩＥ患儿分别采用放射免疫法测定血浆和ＣＳＦ中ＥＴ－１，硝酸根不原酶法测定血浆和ＣＳＦ中ＮＯ＾－２／ＮＯ＾－３值ＮＯ水平，并观察其急性期，恢复期的动态变化。

The study of endothelin and nitric oxide levels in plasma and cerebrospinal fluid of newborn infants with hypoxic ischemic encephalopathy

Objective To study the relationship and the effects of endothelin1 (ET1) and nitric oxide (NO) in plasma and cerebrospinal fluid (CSF) on hypoxic ischemic encephalopathy (HIE) Methods In 35 newborn infants with HIE (10 cases of mild, 13 cases of moderate and 12 cases of severe status), ET1 levels in plasma and CSF were measured by radio immunoassay both in acute and recovery phases; NO2/NO3 level of plasma and CSF were measured with nitrate reductase Results In acute phase, the ET1 levels of plasma and CSF in infants with moderate and severe HIE were much higher than those in controls, especially the severe cases There was no significant difference of ET1 levels between the mild HIE and the control infants However, in recovery phase, the ET1 in severe HIE infants kept at the high level In acute phase the NO levels in plasma and CSF of the moderate and severe HIE infants were much higher than those of the controls The NO levels of the severe HIE infants were obviously higher than those of the mild ones In recovery phase, the NO levels in all three groups of the HIE infants decreased to the normal levels, which were no significant differences in compared with the control Conclusion The brain damage of the HIE infants might relate to the over expression of ET1 and NOThe persistent increase of ET1 in plasma and CSF might be an indicator of a serious illness and a poor prognosis