| 细胞内信号传递对脐血淋巴细胞凋亡的影响 |
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| 引用本文： | 刘恩梅,李成荣,杨锡强.细胞内信号传递对脐血淋巴细胞凋亡的影响[J].中华儿科杂志,1999,37(6):337-339. |
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| 作者姓名： | 刘恩梅 李成荣 杨锡强 |
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| 作者单位： | 重庆医科大学附属儿童医院，深圳市儿童医院 |
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| 基金项目： | 国家自然科学基金,四川省卫生厅科研基金 |
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| 摘 要： | 目的 探讨新生儿Ｔ淋巴细胞内信号传递与脐血淋巴细胞凋亡的关系。方法 以健康成人外周血单个核细胞为对照，测定１９例足月新生儿脐血淋巴细胞内钙离子浓度，蛋白激酶Ａ活性和蛋白激酶激动剂ＰＭＡ对脐血淋巴细胞凋亡的影响。结果 （１）经抗－ＣＤ３刺激前或刺激事脐血单个核细胞和ＰＢＭＣ细胞内钙离子浓度差异无显著意义；（２）ＰＭＡ可纠正抗ＣＤ３诱导的ＣＢＭＣ凋亡；（３０培养２小时，ＣＢＭＣ ＰＫＡ活性自发显著升高
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| 关 键 词： | 胎血 单核细胞 脱噬作用 信号传递 |
The effect of intracellular signal transduction on apoptosis of cord blood mononuclear cells |
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| LIU Enmei,LI Chengrong,YANG Xiqiang,et al..The effect of intracellular signal transduction on apoptosis of cord blood mononuclear cells[J].Chinese Journal of Pediatrics,1999,37(6):337-339. |
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| Authors: | LIU Enmei LI Chengrong YANG Xiqiang |
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| Institution: | LIU Enmei,LI Chengrong,YANG Xiqiang,et al. Childrens Hospital,Chongqing University of
Medical Sciences,Chongqing 400014 |
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| Abstract: | Objective In order to explore the effect of
intracellular signal transduction on apoptosis of cord blood mononuclear cells. Methods
Intracellular calcium concentration, protein kinase A (PAK) activity, and the effect of PMA, which
is the activator of protein kinase C (PKC) on apoptosis of cord blood mononuclear cells (CBMC)
were determined in CBMC with the control of normal adult peripheral blood mononuclear cells
(PBMC). Results There were no differences in calcium concentrations between CBMC and
PBMC before or after stimulation of antiCD3. PMA could completely correct the CBMC apoptosis
induced by antiCD3. Spontanous PKA activities in CBMC cultured for 2 hours in absence of
mitogen increased from 1.99 to 2.37 (P<0.01). While PKA activities in PBMC decreased.
Conclusion The imbalance of intracellular signal transduction might be the main cause of
apoptosis induced by antiCD3 in CBMC. But spontanous apoptosis in CBMC might related to
increased PKA activities. |
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| Keywords: | Tetal bloodLeukocytes mononuclearApoptosisSignal transduction |
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