大鼠■创伤牙周组织中PGE2及TXB2水平的分析

目的:观察大鼠创伤牙周组织中的PGE2、TXB2水平的变化,探讨大鼠创伤对牙周组织的影响。方法:将48只大鼠随机分为实验组和对照组,每大组各又分为4小组,每小组6只,创伤3d、2周、4周及12周时处死大鼠,提取创伤牙龈、牙槽骨标本测定PGE2、TXB2含量。检测结果进行统计学分析。结果:对照组各组间PGE2、TXB2含量均无差异(P>0.05)。实验组各组间PGE2、TXB2含量有显著差异(P<0.001),其中3d组除牙龈TXB2高于对照组外(P<0.01),余PGE2、TXB2均明显高于对照组(P<0.001);2周组PGE2、TXB2最高,4周组PGE2、TXB2有所下降,但均高于对照组(P<0.001);12周组PGE2、TXB2明显下降,接近对照组(P>0.05)。结论:创伤牙周组织中PGE2、TXB2的变化规律是由病变到修复到适应的过程。

Analysis of the PGE2 and TXB2 in Periodontium of the Rat with Occlusal Trauma

Objective：To investigate the changes ofprostaglandin E2（PGE2） and thromboxane B2（TXB2） in pedodontium while the rat with occlusal trauma, and discuss effects on periodontium. Methods： 48 Sprague-Dawley rats were randomly assigned to 8 groups, 4 experimental groups and 4 control groups. Each group had 6 rats. The rats in experimental groups were operated occlusal trauma, not so the control. They were killed at 3days, 2 weeks ,4 weeks and 12 weeks postoperation. The amount of PGE2 and TXB2 were tested in gingiva and alveolar bone between experimental and control groups. Results： The level of PGF2 in control groups was no obvious difference （P〉0.05）, so as the level of TXB2; while those in experimental groups showed significant difference （P〈0.001）. The levels of PGE2 and TXB2 in experimental groups were higher than those in control（P 〈0.001） besides TXB2 in gingiva at 3 days （P 〈0.01）. Those in experimental groups came up to the highest level at 2 weeks, coming down at 4 weeks. The levels in experimental groups decreased and were close to those in control at 12 weeks （P 〉0.05）. Conclusion： The changes ofperiodontium in occlusal trauma is from pathological changes to repair and adaptation.