海南省儿童非脊髓灰质炎肠道病毒感染的研究

目的　为了解海南省儿童非脊髓灰质炎肠道病毒 (NPEV)的感染状况 ,为我省今后进一步研究肠道病毒对人类健康的影响提供本底资料。方法　采集不同组群儿童粪便标本 ,采用WHO提供的RD、HEP -2、L2 0B细胞进行病毒分离和鉴定 ,并对结果进行比较分析。结果　对 1994～ 2 0 0 1年采集的 10 2 6例儿童的粪便标本进行病毒分离和鉴定 ,NPEV分离率为 19.79% ,其中急性弛缓性麻痹 (AFP)病例组NPEV分离率最高 ,为 2 6.5 8% ,密切接触者和健康儿童中粪便标本的NPEV分离率分别为 17.37%和 2 0 .41% ,AFP病例与接触者NPEV分离率有显著性差异 (x2 =9.468,P <0 .0 1)。不同组群儿童感染的NPEV型别差异较大。不同型别NPEV毒株在海南省AFP病例、AFP接触者和健康儿童中感染的机率有所差别。有些型别的NPEV在三组儿童中都有循环 ,但有些型别的NPEV毒株仅从AFP病例或其接触者中分离到。结论　在我国实现消灭脊髓灰质炎的目标后 ,开展儿童NPEV感染状况的研究能为进一步证实和巩固消灭脊灰成果提供科学依据 ,并对我国深入研究NPEV对人类健康的影响有十分重要的意义

Studies on non- polio enterovirus infection in children in Hainan Province

Objective In order to know infectious situation of Non-polio enterovirus(NPEV) in different children groups in Hainan province to provide basic data for research of NPEV to people health.Methods Stool samples which were collected from different children groups were isolated and identified NPEV strains with RD,HEP-2 and L20B cell lines provided by WHO.The results were compared and analyzed.Results Stool samples of 1026 children were tested for isolating and identifying NPEV.The NPEV isolation rate was 19.79%.That of AFP cases was the highest one,26.58%.Rate of AFP cases contacts and healthy children group were respectively 17.37%,and 20.41%.There was significant difference between that of AFP cases and AFP cases contacts(x 2=9.468,P<0.01). The types of NPEV existed great differences in different children groups.The infection rates of different type NPEV strains were different in each children group in Hainan province.Some types of NPEV were circulated among all groups.But some types of NPEV were only isolated from AFP cases or AFP contacts.Conclusion After achieving the goal of poliomyelitis eradication in our country study of NPEV infection situation could provide scientific basis to ensure and enhance achievement of poliomyelitis eradication.It was very important to study NPEV to people health in our country.