| Troglitazone, a peroxisome proliferator-activated receptor γ ligand, induces growth inhibition and apoptosis of HepG2 human liver cancer cells |
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| 作者单位： | Yan-Ming Zhou（Department of Molecular Oncoiogy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China） ;Yin-Hao Wen（Department of Molecular Oncoiogy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China） ;Xiao-Yan Kang（Department of Molecular Oncoiogy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China） ;Hai-Hua Qian（Department of Molecular Oncoiogy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China） ;Jia-Mei Yang（Department of Molecular Oncoiogy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China） ;Zheng-Feng Yin（Department of Molecular Oncoiogy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China） ; |
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| 基金项目： | Supported by Grants from the State Key Basic Research Program, No. 2002CB513100 |
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| 摘 要： | AIM： To examine the effect of troglitazone, a peroxisome proliferator-activated receptor γ （PPARγ） ligand, on the proliferation and apoptosis of human liver cancer cells. METHODS： Liver cancer cell line HepG2 was cultured and treated with troglitazone. Cell proliferation was detected by 3-（4-,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide （MTT） assay; apoptosis was detected by flow cytometry and terminal deoxynucleotidyl transferase- mediated nick end labeling of DNA fragmentation sites （TUNEL） assay; and apoptosis-related protein was detected by immunocytochemistry and Western blotting. RESULTS： Troglitazone inhibited growth and induced apoptosis of HepG2 cells in a dose-dependent manner, and induced activation of caspase-3 expression. Troglitazone not only drove apoptosis-inhibiting factor survivin to translocate incompletely from the nucleus to the cytoplasm, but also inhibited expression of survivin, while it did not affect expression of apoptosis-promoting factor Bax. CONCLUSION： PPARγ ligands inhibit growth and induce apoptosis of liver cancer cells, and may have applications for the prevention and treatment of liver cancer.
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| 关 键 词： | 肝肿瘤 细胞凋亡 过氧物酶体 治疗方法 |
| 收稿时间： | 2007-09-19 |
Troglitazone, a peroxisome proliferator-activated receptor �� ligand, induces growth inhibition and apoptosis of HepG2 human liver cancer cells |
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| Yan-Ming Zhou Yin-Hao Wen Xiao-Yan Kang Hai-Hua Qian Jia-Mei Yang Zheng-Feng Yin. Troglitazone, a peroxisome proliferator-activated receptor �� ligand, induces growth inhibition and apoptosis of HepG2 human liver cancer cells[J]. World journal of gastroenterology : WJG, 2008, 14(14): 2168-2173. DOI: 10.3748/wjg.14.2168 |
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| Authors: | Yan-Ming Zhou Yin-Hao Wen Xiao-Yan Kang Hai-Hua Qian Jia-Mei Yang Zheng-Feng Yin |
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| Affiliation: | Department of Molecular Oncology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China |
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| Abstract: | AIM: To examine the effect of troglitazone, a peroxisome proliferator-activated receptor γ (PPARγ) ligand, on the proliferation and apoptosis of human liver cancer cells.METHODS: Liver cancer cell line HepG2 was cultured and treated with troglitazone. Cell proliferation was detected by 3-(4-,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay; apoptosis was detected by flow cytometry and terminal deoxynucleotidyl transferasemediated nick end labeling of DNA fragmentation sites (TUNEL) assay; and apoptosis-related protein was detected by immunocytochemistry and Western blotting.RESULTS: Troglitazone inhibited growth and induced apoptosis of HepG2 cells in a dose-dependent manner,and induced activation of caspase-3 expression.Troglitazone not only drove apoptosis-inhibiting factor survivin to translocate incompletely from the nucleus to the cytoplasm, but also inhibited expression of survivin,while it did not affect expression of apoptosis-promoting factor Bax.CONCLUSION: PPARγ ligands inhibit growth and induce apoptosis of liver cancer cells, and may have applications for the prevention and treatment of liver cancer. |
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| Keywords: | Peroxisome proliferator-activated receptor γ Troglitazone Liver neoplasms Apoptosis |
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