硝喹对大白鼠体内约氏疟原虫红细胞外期发育的影响

大白静脉内接种子孢子(SP)后24小时(h),灌服硝喹醋酸盐(1mg/kg)单次剂量,在不同时间活检或活杀动物,检查红外期原虫,其中部分动物并作血检,观察原虫血症。结果。①接种SP后48h,给药组动物肝组织内原虫密度较低,退变原虫发生率高;54、65和72h红外期原虫核大小不等,与原虫体积不相称,核团块大小不一,数量增多;并可见到白细胞浸润的变性原虫;在接种SP后72h内,均未发现成熟红外期原虫。②给药组8只动物中仅一只血检阳性。但密度低,虫现前斯长。实验结果表明硝喹对约氏疟原虫具有病因性预防效果,并提示硝喹可能通过抑制核分裂而影响红外期原虫的发育。

Effects of Nitroquine on the Development of Exoerythrocytic Forms of Plasmodium yoelii yoelii in Rats

Wistar rats -were given orally a single dose of nitroquine acetate ( 1 mg/kg) 24 hours after an intravenous inoculation of the sporoizoites of Plasmodium yoelii yoelii strain BY 265. Liver biopsies were taken and some animals were killed at regular intervals to observe the exoerythrocytic forms(EEF). Blood smears of some animals were made to observe the parasitemia. The results were , as follows.1. The density of the EEF in the livers of the treated animals was low but the number of degenerated EEF was high 48 hours after inoculation. At this time, the EEF was smaller in size with less number of nucleus, but with irregular nuclear masses. The EEF at 54, 65 and 72 hours were characterized by un- even size of the nuclei, which were disproportionate to the size of the parasites, and by much more irregular masses of nucleus. Some degenerated EEF infiltrated by WBC could be found. There was no matured tissue schizont within 72 hours after sporozoile inoculation.2. Only 1 out of the 8 treated animals became patent of parasitemia of low level with a prolonged prepatent period.The results indicate that nitroquine exerts a causal prophylactic effect on rodent malaria and interferes with the development of EEF by suppressing the ouclear division