| MTT法观察补肾健骨汤对大鼠成骨细胞增殖的影响 |
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| 引用本文： | 谈志龙 邢国胜 王淑云 白人骁. MTT法观察补肾健骨汤对大鼠成骨细胞增殖的影响[J]. 中国骨质疏松杂志, 2004, 10(1): 94-95,102 |
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| 作者姓名： | 谈志龙 邢国胜 王淑云 白人骁 |
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| 作者单位： | 300211,天津市天津医院 |
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| 摘 要： | 目的采用MTT法观察中药补肾健骨汤载药血清对大鼠成骨细胞增殖的影响.方法将补肾健骨汤口饲大鼠,每日1次,连续7 d,于末次给药后2 h采血,分离获得含药血清,加入成骨细胞培养液中,连续培养10 d,从第2天开始用MTT法测定光密度值,探讨该方剂对成骨细胞增殖的影响.结果大鼠成骨细胞体外培养在对数生长期第3天至第9天载药血清组明显高于对照组.结论用MTT法观察补肾健骨汤载药血清对大鼠成骨细胞增殖有明显促进作用.
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| 关 键 词： | MTT比色法 中药 成骨细胞 骨质疏松 |
| 修稿时间： | 2003-06-25 |
Effects of Bushen Jiangu decoction on proliferation of rat osteoblast assessed by MTT method |
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| TAN Zhi-long,XING Guosheng,WANG Shuyun,et al.. Effects of Bushen Jiangu decoction on proliferation of rat osteoblast assessed by MTT method[J]. Chinese Journal of Osteoporosis, 2004, 10(1): 94-95,102 |
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| Authors: | TAN Zhi-long XING Guosheng WANG Shuyun et al. |
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| Affiliation: | TAN Zhi-long,XING Guosheng,WANG Shuyun,et al. Tianjin Hospital,Tianjin 300211,China |
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| Abstract: | Objective To study the effects of Bushen Jiangu decoction on the proliferation of osteoblast observed by MTT method. Methods Rats were fed with Bushen Jiangu decoction once per day for 7 days. Blood was collected 2 hours after the last administration of medicine. Then serum was separated and added to the osteoblast culture medium. After 10-day culture,the absorbence density was determined by MTT method to study the effects of this medicine. Results In logarithmic growth period, the MTT value of rat osteoblast cultured with the serum was significantly higher than that in control group from day 3 to day 9. Conclusion Bushen Jiangu decoction promotes rat osteoblast proliferation, which can be detected by MTT method. |
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| Keywords: | MIT method Traditional Chinese medicine Osteoblast Osteoporosis |
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