肿节风对鼻咽癌裸鼠移植瘤凋亡和端粒酶活性的影响

目的:评价肿节风提取物诱导鼻咽癌裸鼠移植瘤细胞凋亡作用和抑制端粒酶活性的能力。方法:将移植人鼻咽癌CNE1、CNE2细胞的裸鼠随机分为肿节风组、环磷酰胺组和对照组,定期检测裸鼠的体重及肿瘤体积,给药一个疗程(30d)后解剖获取肿瘤组织,称量瘤体重量,计算抑瘤率。运用透射电镜观察瘤组织细胞超微结构的改变;免疫组织化学法检测瘤组织Bcl-2和Bax的表达;流式细胞仪进行细胞周期、细胞凋亡分析;TUNEL法检测细胞凋亡;TRAP-ELISA方法观察药物处理前后组织端粒酶活性的变化。结果:动物实验表明,肿节风对CNE1、CNE2移植瘤有明显的抑瘤作用,抑瘤率分别为40.8%和46.8%(与对照组比较P<0.01);且Bcl-2蛋白表达明显低于对照组(P<0.01),而Bax蛋白表达明显高于对照组(P<0.01);电镜下肿节风组瘤组织中可见较多典型的凋亡形态学改变;TUNEL法肿节风组凋亡指数明显高于对照组(P<0.01);流式细胞技术显示肿节风组G0/G1期细胞高于对照组(P<0.01),细胞阻滞在G0/G1期,凋亡率明显高于对照组(P<0.01);端粒酶活性检测中可见肿节风组比对照组端粒酶活性降低(P<0.01)。结论:肿节风在体内具有抑制鼻咽癌裸鼠移植瘤生长的作用,其抑瘤作用机制与诱导凋亡有关,抑制端粒酶活性可能起部分作用。

The inhibitory role of zhongj iefeng extracts on apoptosis and telomerase activity of nasopharyngeal carcinoma cell line exnograf in nude mice

Objective:This study was designed to evaluate the effect of zhongjiefeng extracts on telomerase activity and apoptosis of implanted human nasopharyngeal carcinoma cell lines in nude mice.Method:Nude mice with imp lanted human nasopharyngeal carcinoma cell lines CNE1 and CNE2 were randomly divided into three groups,which were the group of zhong-jiefeng,normal control group,and CTX therapy group.The weight of nude mice and the volume of tumor were regularly measured.After a course of treatment(30 d),tumor tissues were obtained with autopsy,and then they were weighed to calculate the inhibitive rate of the growth of tumor.The changes of ultra micro-structure of NPC cells were observed by transmission electron microscope.Expressions of Bcl-2 and Bax of xenografts were investigated by immunohistochemistry.The apoptosis of CNE1 and CNE2 cells was observed using TUNEL and FACS with PI-staining.The telomerase activity was observed by TRAP-ELISA method.Result:The zhongjiefeng extracts significantly inhibit the growth of tumor compared to normal control group.The inhibitory rate were 40.8%(P<0.01) and 46.8%(P<0.01),respectively.The expression of Bcl-2 was lower(P<0.01) and the expression of Bax was higher(P<0.01) in the zhongjiefeng group than that in normal control group.Electron microscopy indicated the typical apoptosis of tumor cells,such as marginal nuclei,chromatin condensation and nuclei fragmentation,and apoptotic bodies.As result of the flow cytometry showed,the exhibition rate of apoptosis of cell in the group treated with zhongjiefeng was higher than that of normal control group(P<0.01)in G0/G1 phase.Zhongjiefeng arrested cells in G0/G1 phase of the cell cycle.The telomerase activity of zhongjiefeng and CTX group was lower than that of the normal group(P<0.01).Conclusion:Zhongjie feng suppressed the growth of tumor in vivo.The anticancer effects of zhongjiefeng were associated with the induction of apoptosis and partly with the suppression of telomerase activity.