脐带间充质干细胞减轻哮喘小鼠气道炎症的研究

目的研究脐带间充质干细胞对鸡卵清蛋白诱导的哮喘小鼠模型气道炎症的抑制作用。方法将48只6⁸周龄雌性BALB/c小鼠随机分为四组,单纯哮喘组(OVA组),干细胞治疗组(MSC组),地塞米松治疗组(DM组)及生理盐水对照组(NS组)。23 d后,支气管肺泡灌洗液(BALF)细胞计数总细胞数、嗜酸粒细胞数;取肺组织行病理切片HE染色观察气道周围炎细胞浸润情况。同时免疫组织化学法观察IL-17蛋白在各组小鼠肺组织中的表达。结果同其他各组相比,单纯哮喘组肺泡灌洗液和肺组织中有大量炎性细胞浸润,而地塞米松治疗组、干细胞治疗组气道炎细胞浸润明显减轻(P<0.05),两个治疗组之间无明显差异(P>0.10)。与此结果相一致,MSC组、DM组中,炎性因子IL-17表达较OVA组明显减少(P<0.05),而DM组IL-17表达与MSC组相比无明显差异(P>0.10)。结论脐带间充质干细胞能够明显抑制鸡卵清蛋白诱导的哮喘小鼠气道炎症。

Suppression of Allergic Airway Inflammation in a Mouse Model of Asthma by Exogenous Umbilical Cord Mesenchymal Stem Cells

Objective To study the Inflammation inhibition effect of umbilical cord mesenchymal stem cells transplantation on the asthmatic mice induced by chicken ovalbumin. Methods Forty male BALB/c mice were equally randomized into four groups. The asthma group; the ucMSC intervention group; the dexamethasone treatment groups and the blank control group. Mice were sacrificed on day 23. BALF were obtained and centrifuged to pellets and super natants. The total number of cells and the eosinophils in BALF was counted. The paraffin embedded sections of lung tissue were stained with HE. The expression of IL-17 in all groups were detected by immunohistochemical method. Results Compared with the other groups, there were a great number of inflammatory cell infiltrations in the asthma group. However, there was no obvious inflammatory cells infiltration in ucMSCs control group and the dexamethasone treatment groups（P〈0.05）. Compared with ucMSC intervention group, the inflammatory cells infiltration decreased remarkably in dexamethasone treatment groups. Consistent with the above results, the expression of IL-17 were reduced in the ucMSC intervention group and dexamethasone treatment groups, and more obvious in dexamethasone treatment groups. Conclusion Airway inflammation of the mice induced by chicken ovalbumin were significantly reduced by exogenous ucMSC.