营养性肥胖对大鼠精子的影响

目的:观察营养性肥胖对雄性大鼠睾丸生精功能的影响。方法:健康青春期SD雄性大鼠20只,按随机化原则分为两组,每组10只,正常对照组给予普通饲料喂养,高脂饮食组用高脂、高热量饲料喂养12周,取附睾精子观察精子密度、活力,光镜下观察睾丸组织显微结构的变化。结果:高脂饮食组精子密度、活动率、前向运动精子、快速前向运动精子百分比均有不同程度的降低(P<0.01),睾丸组织的显微结构改变。结论:营养性肥胖影响了大鼠睾丸的发育,精子密度、活力都下降。

Effects of obesity on sperm in diet-induced obesity rats

Objective:To observe the effects of alimentary obesity on gonepoiesis of the male rats by diet-induced obesity.Methods: Twenty adolescent male Sprague-Dawley rats were evenly randomized into two groups.The control group were prescribed with usual food,but fat diet group were fed with fat-enriched diet for 12 weeks.At the end of the experiment,sperm taken from epididymides were observed by light microscope for determining sperm density,motility,progressive motility,fast progressive motility and the change of microstructure of testicle tissues.Results:In fat diet group,sperm density,motility,progressive and fast progressive motility were significantly lower than that of control group(P<0.01) with changes of the microstructure to a certain degree.Conclusion: Fat-enriched diet can produce effects on the development of testicle.