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胡枝子醇提工艺的研究
引用本文:易延逵,邓虹蛛,张荣幸,杨永华.胡枝子醇提工艺的研究[J].中医药学刊,2007,25(12):2644-2646.
作者姓名:易延逵  邓虹蛛  张荣幸  杨永华
作者单位:[1]南方医科大学,广东广州510515 [2]湖南省中医药研究院,湖南长沙410006
摘    要:以总黄酮量为指标,对胡枝子醇提浓度进行选择试验;考查了水、40%乙醇、55%乙醇、70%乙醇、85%乙醇提取液中总黄酮的提取量,采用紫外分光光度法测定总黄酮含量,并绘制了曲线图;结果表明胡枝子醇提以55%-60%乙醇提取为佳。以总黄酮量、浸膏得率为指标,对胡枝子醇提工艺参数进行选择试验;采用正交试验法考查了加醇量、提取次数、提取时间对提取液中总黄酮提取量、浸膏得率的影响,采用紫外分光光度法测定总黄酮含量,并进行了验证试验;结果表明胡枝子醇提以55%一60%乙醇回流提取2次,第1次加7倍量,回流2.0h。第2次加5倍量水,回流1.5h提取为佳。

关 键 词:胡枝子  正交试验法  提取工艺  总黄酮  UV法
文章编号:1673-7717(2007)12-2644-03
收稿时间:2007-07-09

Study on the Ethanol Extraction Process of Lespdeza bicolor Turcz
YI Yan-kui , DENG Hong-zhu ,ZHANG Rong-xing , YANG Yong-hua.Study on the Ethanol Extraction Process of Lespdeza bicolor Turcz[J].Study Journal of Traditional Chinese Medicine,2007,25(12):2644-2646.
Authors:YI Yan-kui  DENG Hong-zhu  ZHANG Rong-xing  YANG Yong-hua
Abstract:Take the total flavoues as referance, select the rate of ethanol of the extraction of Herba Lespdeza. , to dewell on the extracting quanitity of total flavoues in the water, 40% ethanol, 55% ethanol, 70% ethanol, 85% ethanol extracting solution, applying UV method to determine the total flavoues content, moreover, describe the curve tab. The result showed that the optimum extracting rate of ethanol of Herba Lespdeza. is 55% - 60%. Take the total flavoues as referance, select the rate of ethanol of the extraction of Lespdeza bicolor Turcz, applying Orthogonal Design method to dewell on the extracting quanitity of total flavoues in the water, 40% ethanol, 55% ethanol, 70% ethanol, $5% ethanol extracting solution, applying UV method to determine the total flavoues content, moreover, describe the curve tab. The result showed that the optimum extracting rate of ethanol of Lespdezabicolor Turcz is 55% - 60%.
Keywords:Lespdeza bicolor Turcz  Orthogonal Design method Extraction Process  total flavoues  UV method
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