| 人膀胱移行细胞癌细胞系PUMC—91细胞HPV—18 DNA的检测 |
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| 引用本文： | 何祖根,郑闪,刘海涛,王顺宝.人膀胱移行细胞癌细胞系PUMC—91细胞HPV—18 DNA的检测[J].实用癌症杂志,2001,16(3):225-227. |
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| 作者姓名： | 何祖根 郑闪 刘海涛 王顺宝 |
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| 作者单位： | 100021,中国医学科学院中国协和医科大学肿瘤研究所,肿瘤医院病理科 |
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| 基金项目： | 本课题受国家自然科学基金资助(编号39370291) |
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| 摘 要： | 目的 探讨膀胱移行细胞癌与人乳头状瘤病毒（HPV）感染之间的关系。方法 采用细胞培养、PCR张Southern Blot杂交方法，对膀胱移行细胞癌细胞系PUMC－91（1991年建立）细胞的人乳头状瘤病毒（HPV）DNA进行检测。结果 人膀胱移行细胞癌细胞系PUMC－91细胞内含有整合在细胞内含有整合在细胞基因组DNA上的不完整HPV－18DNA。结论 HPV－18感染与膀胱移行细胞癌的发生可能有一定关系，PCMC－91细胞系是研究这一关系的良好体外模型。
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| 关 键 词： | 膀胱移行细胞癌细胞系 人乳头状瘤病毒 聚合酶链反应 膀胱癌 |
| 文章编号： | 1001-5930(2001)03-0225-03 |
| 修稿时间： | 2000年11月13 |
Detection of Human Papillomaviruses-18 DNA in Transitional Cell Carcinoma Cell Line of PUMC-91 |
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| HE Zu gen,ZHENG Shan,LIU Hai tao,et al..Detection of Human Papillomaviruses-18 DNA in Transitional Cell Carcinoma Cell Line of PUMC-91[J].The Practical Journal of Cancer,2001,16(3):225-227. |
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| Authors: | HE Zu gen ZHENG Shan LIU Hai tao |
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| Abstract: | Objective To detected the human papillomaviruses(HPV) DNA in PUMC 91,a cell line of transitional cell carcinoma of human urinary bladder,which has been established in the department of pathology of PUMC hospital in 1991.MethodsCell culture,PCR and Southern Blot hybridization were used.Results An integrated and incomplete HPV DNA of type 18 was found in PUMC 91.Conclusion There may have some relationship between HPV 18 infection and TCC in bladder,and PUMC 91 cell line is a model in vitro to research the relationship. |
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| Keywords: | Transitional cell carcinoma cell line Human papillomaviruses(HPV) PCR Southern Blot hybridization |
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