硝普钠治疗大鼠应激性胃黏膜损伤的实验研究

①目的　观察外源性一氧化氮 (NO)供体硝普钠对大鼠应激性胃溃疡的治疗作用。②方法　采用浸水束缚应激建立应激性胃溃疡模型 ,应用多普勒血流仪测定硝普钠治疗后胃黏膜血流量 (GMBF) ,观察胃黏膜损伤分数、血清和胃黏膜NO、ET含量的变化。③结果　大鼠浸水束缚应激可以引起胃黏膜严重损伤 ,GMBF明显下降 ,NO含量降低和ET含量增加 (F =2 .882～ 1 8.5 5 5 ,q =2 .4 2 3～ 6 .5 74 ,P <0 .0 5、0 .0 1 )。治疗 5d后 ,硝普钠使胃黏膜损伤基本愈合 ,胃黏膜NO含量明显升高 ,ET含量显著减小 (F =3.384～ 5 .4 97,q =2 .4 88～ 3.90 5 ,P <0 .0 5 )。④结论　硝普钠对应激性胃溃疡有明显的治疗作用

AN EXPERIMENTAL STUDY ON THE EFFECTS OF SODIUM NITROPRUSSIDE ON STRESS-INDUCED GASTRIC MUCOSA INJURIES IN RATS

Objective To observe the therapeutic effects of Sodium Nitroprusside, the donor of exogenous NO, for stress ulcer of the stomach in rats. Methods A stress ulcer model of the stomach in rats was established by soaking rats in water and binding their feet. The changes of the damage rate, the volume of blood flow, the levels of NO, and ET in gastric mucosa were observed. Results The gastric mucosa of the bound and soaked was injured seriously, and the volume of blood flow was decreased distinctively. The NO level in gastric mucosa decreased, but that of ET increased ( F=2.882-18.555, q=2.423-6.574, P < 0.05 ,0.01). After five days of treatment with Sodium Nitroprusside, the injuries in gastric mucosa were basically cured. The NO level in gastric mucosa increased, while that of ET decreased significantly ( F=3.384-5.497, q=2.488-3.905, P <0.05). Conclusion Exogenous NO can effectively treat stress ulcer of the stomach.