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高效液相色谱法测定不同产地黄芩中黄酮化合物的含量
引用本文:杨立新,刘岱,冯学锋,崔淑莲,杨京玉,唐晓军,何希荣,刘菊福,胡世林.高效液相色谱法测定不同产地黄芩中黄酮化合物的含量[J].中国中药杂志,2002,27(3):166-170.
作者姓名:杨立新  刘岱  冯学锋  崔淑莲  杨京玉  唐晓军  何希荣  刘菊福  胡世林
作者单位:中国中医研究院,中药研究所,北京,100700
基金项目:国家自然科学基金重点资助项目 ( 39730 5 0 0 )
摘    要:目的 :测定不同产地黄芩中黄酮类化合物的含量 ,以评价其道地性。方法 :高效液相色谱法 (HPLC)。结果 :17个产地 25份样品中黄芩苷含量变化在 6%~19%、汉黄芩苷在 2%~8%、黄芩素在 0.1%~1.6%、黄芩新素在 0.01%~0.2%、汉黄芩素在 0.01%~0.3% ,粘毛黄芩素、木蝴蝶素为痕迹量或未检出。结论 :单纯以黄芩苷的含量来评价其质量是不够的 ,建议将黄芩苷与汉黄芩苷的含量比值在 3以下、黄芩苷与黄芩素、黄芩苷与汉黄芩素的含量比值在 20~50等参数列为质量控制指标。

关 键 词:黄芩  黄芩苷  汉黄芩苷  黄芩素  汉黄芩素  黄芩新素
文章编号:1001-5302(2002)03-0166-05
收稿时间:2001/12/30 0:00:00
修稿时间:2001年12月30

Detrmination of Flavone for Scutellaria baicalensis from Different Areas by HPLC
YANG Li-xin;LIU Dai;FENG Xue-feng;CUI Shu-lian;YANG Jing-yu;TANG Xiao-jun;HE Xi-rong;LIU Ju-fu;HU Shi-lin.Detrmination of Flavone for Scutellaria baicalensis from Different Areas by HPLC[J].China Journal of Chinese Materia Medica,2002,27(3):166-170.
Authors:YANG Li-xin;LIU Dai;FENG Xue-feng;CUI Shu-lian;YANG Jing-yu;TANG Xiao-jun;HE Xi-rong;LIU Ju-fu;HU Shi-lin
Institution:Institute of Chinese Materia Medica, China Academy of TCM, Beijing 100070, China.
Abstract:OBJECTIVE: To describe the difference between native and nonative herbs by determining contents of seven kinds of flavone for twenty-five samples from seventeen areas. METHODS: HPLC. Fluid phase: MEOH-H2O-CH3COOH(ICE) (41:59:0.2) and (50:50:0.2). Detection wavelength: 275. RESULTS: The contents of baicalin are 6%-9%, wogenin are 2%-8%, baicalein are 0.1%-1.6%, neobaicalein are 0.01%-0.2%, wogonin are 0.01%-0.3%, visidulin and oroxylin are trace amounts or undetected. CONCLUSION: The native and nonative herbs have no distinct differce in absolute component ratio. The ratio of baicalin and wogenin is under three. The ratio of baicalin and baicalein, baicalin and wogonin is between twenty and fifty.
Keywords:Scutellaria baicalensis  baicalin  wogenin  baicalein  wogonin  neobaicalein
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