载脂蛋白H基因多态性与冠心病及血脂代谢关系的研究

目的探讨载脂蛋白H(ApoH)外显子3、8基因多态性与冠心病(CHD)、血脂代谢的关系。方法采用聚合酶链式反应-单链构象多态技术(PCR-SSCP)分析方法,分析了100例健康人及110例CHD患者的ApoH外显子3,8基因型及血脂测定。结果(1)CHD组外显3 GG基因型的频率为81.8%,GA+AA基因型频率为18.2%,G等位基因频率为88%,A等位基因频率是12%,与对照组比较无差异。(2)CHD组外显子8 GG基因型频率为74.5%,GC基因型频率为25.5%,G等位基因频率为87%,C等位基因频率为13%,与对照组比较CHD组的GC基因型频率及C等位基因频率显著增高。(3)外显子3 CHD组低密度胆固醇(LDL-C)高于对照组(P<0.05),CHD组及对照组各基因型间的血脂水平无差异;(4)外显子8 CHD组LDL-C也显著高于对照组(P<0.05),CHD组GC基因型的甘油三酯(TG)显著高于GG型和及对照组的各基因型。结论(1)ApoH外显子3基因多态性与CHD及血脂代谢无相关性;(2)ApoH外显子8 GC基因型及C等位基因与CHD有关,ApoH外显子8基因多态性与TG有关。

Relationship between apolipoprotein H gene polymorphism with coronary heart disease

Objective To investigate relationship between the apolipoprotein H(ApoH) exon3 and exon 8 polymorphism with coronary heart disease(CHD).Methods Polymerase chain reaction and technique of restriction fragment length polymorphism were used to determine the genotypes of ApoH exon 3 and exon8 in 110 patients with CHD and 100 health control group.Results The genotype GG of ApoH in the CHD group was 81.8%,GA AA genotype was 11.8% only,the frequency of G allele was 88%,while 12%in the A allele,there was no significant difference with the control groups,the frequency of GG genotype in ApoH exon 8was74.5%,GC genotype was25.5%,the frequency of G allele was 87%,the frequency of GC genotype and C allele in CHD group were significant high than that in control group.LDL level in CHD group was significant high than that in control group(P<0.05).LDL level of exon 3 in CHD group was significant high than that in control group(P<0.05).But there were no significant difference among the level of lipid in the genotype of CHD and in the control group.LDL level of exon 8 in CHD group was significant high than that in control group(P<(0.05).)TG level in genotype GC of CHD was significant high than that either in GG genotype of CHD or in any genotype of the control group.Conclusion The polymorphism of the apolipoprotein H exon 3 has no relationship with CHD,but the GC genotype of ApoH exon 3 and C allele have close relation with CHD and serum TG level.The polymorphism of the apolipoprotein H exon 3 has no significant relationship with the level of serum lipid.But there are close relation between polymorphism of the apolipoprotein H exon 8 and serum TG level.