| 乙肝表面抗原核酸疫苗在小鼠体内的阳性表达 |
| |
| 引用本文： | 温剑平,杨贵贞.乙肝表面抗原核酸疫苗在小鼠体内的阳性表达[J].中国免疫学杂志,2002,18(3):159-161. |
| |
| 作者姓名： | 温剑平 杨贵贞 |
| |
| 作者单位： | 吉林大学基础医学院免疫教研室,长春,130021 |
| |
| 摘 要： | 目的 :用乙肝表面抗原核酸疫苗免疫小鼠 ,探讨其在体内的表达。方法 :用含乙肝表面抗原基因的质粒载体肌注小鼠 ,免疫组化法检测HBsAg在注射部位肌组织、局部淋巴结、脾脏的表达 ;进一步用ELISA法检测血清中抗HBsAg抗体 ;脾淋巴细胞特异及非特异性增殖试验。结果 :一次免疫后 ,在注射部位肌组织可见HBsAg阳性表达 ;二次免疫后 ,在脾、局部淋巴结可见HBsAg阳性染色。血清抗体出现阳性 ;特异性抗原及ConA均可刺激脾淋巴细胞增殖。结论 :乙肝核酸疫苗肌注小鼠后 ,在骨骼肌、淋巴结、脾脏组织中HBsAg呈阳性表达 ,并出现了一定免疫应答效应。
|
| 关 键 词： | 乙型肝炎 核酸疫苗 表达 |
| 文章编号： | 1000-484X(2002)03-0159-03 |
Expression of hepatitis B virus DNA vaccine in immunized miceWEN Jian-Ping,YANG Gui-Zhen.Immunology Department,School of Basic Medicine of Jilin University,Changchun 130021 |
| |
| WEN Jian-Ping,YANG Gui-Zhen.Immunology.Expression of hepatitis B virus DNA vaccine in immunized miceWEN Jian-Ping,YANG Gui-Zhen.Immunology Department,School of Basic Medicine of Jilin University,Changchun 130021[J].Chinese Journal of Immunology,2002,18(3):159-161. |
| |
| Authors: | WEN Jian-Ping YANG Gui-ZhenImmunology |
| |
| Institution: | WEN Jian-Ping,YANG Gui-Zhen.Immunology Department,School of Basic Medicine of Jilin University,Changchun 130021 |
| |
| Abstract: | Objective:To research the expression of HBsAg DNA vaccine in immunized mice.Methods:The expression of HBsAg DNA vaccine was detected by immunohistochemistry staining in muscle fibers,lymphoid nodes and spleens of mice after intramuscular injection.Anti-HBsAg antibody was assayed by ELISA and the proliferation of spleen cells was measured by MTT.Results:The positive expression was observed in the tissues mentioned above.The titer of serum antibody showed positive results.Spleen cells could be proliferated by stimulating with HBsAg or ConA.Conclusion:The positive expression and certain kinds of immune responses have been shown in immunized mice. |
| |
| Keywords: | Hepatitis B DNA vaccine Expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
