mTOR信号通路对扩张性心肌病大鼠心肌纤维化进程调控作用的实验研究

目的探讨哺乳类雷帕霉素靶蛋白（mTOR）信号通路在扩张性心肌病大鼠心肌纤维化进程中的作用。方法Wistar大鼠30只随机分为2组，扩张性心肌病模型组（DCM组）和对照组，每组15只，DCM组即实验起始每天通过尾静脉注射阿霉素建立大鼠扩心病模型（2．5mg／kg，2次／周，共6周），对照组同法注射等量0．9％氯化钠溶液。6周后超声心动图检测大鼠左心室结构和功能，苦味酸一天狼星红染色观察大鼠心肌间质胶原纤维的变化，RT—PCR方法检测roTOR与真核起始因子4E（eIF-4E）的表达。结果与对照组比较，DCM组大鼠左心室直径增加，收缩功能降低（P〈0．05）；左心室心肌间质胶原容积、mTOR和eIF4E的mRNA表达水平明显增加（P〈0．05）。结论mTOR和eIF-4E的表达增加可能是介导扩张性心肌病心肌纤维化重构的机制之一。

The regulatory role of mTOR/eIF-4E signal transduction pathway during myocardial fibrosis of rats with dilated cardiomyopathy

Objective To investigate the role of mTOR signal transduction pathway during myocardial fibrosis of rats with dilated cardiomyopathy.Methods Thirty adult Wistar rats were randomly divided into two groups,dilated cardiomyopathy model(DCM) group(n=15) and control group(n=15).The animal models of dilated cardiomyopathy were established in DCM group by caudal vein injection with adriamycin at a dose of 2.5 mg/kg,twice a week for 6 weeks,however,the rats in control group received an equivalent volume of 0.9% sal...