| 电纺PLGA/HA复合支架的细胞相容性研究 |
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| 引用本文： | 张华林,陈治清,李咏梅,冯佳.电纺PLGA/HA复合支架的细胞相容性研究[J].哈尔滨医药,2011,31(5):321-322. |
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| 作者姓名： | 张华林 陈治清 李咏梅 冯佳 |
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| 作者单位： | 1. 宁夏医科大学口腔医学院,宁夏银川,750004
2. 四川大学华西口腔医学院,四川成都,610041 |
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| 基金项目： | 教育部科学技术研究重点项目(211196); 宁夏自然科学基金项目(NZ11124); 宁夏医科大学特殊人才科研启动项目(XT201006) |
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| 摘 要： | 目的通过静电纺丝的方法制备PLGA/HA复合支架并对其细胞相容性进行表征。方法先通过高压静电纺丝的方法制备PLGA/HA复合生物支架,再将大鼠的骨髓间充质干细胞接种于复合支架上培养,采用SEM观察细胞在支架上的形貌、MTT法分析细胞的增殖情况。结果 PLGA/HA复合支架是由超细纤维构成的三维多孔结构,与天然的细胞外基质结构相似,骨髓间充质干细胞在其表面附着、铺展良好,HA纳米颗粒的加入明显提高了细胞的粘附率。结论静电纺丝法制得的PLGA/HA复合支架有可能作为骨组织再生的支架在组织工程领域发挥作用。
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| 关 键 词： | PLGA HA 静电纺丝 细胞相容性 |
Study on Cell Compatibility of Electrospun PLGA/HA Composite Scaffolds |
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| ZHANG Hualin,CHEN Zhiqing,LI Yongmei,FENG Jia.Study on Cell Compatibility of Electrospun PLGA/HA Composite Scaffolds[J].harbin medical journal,2011,31(5):321-322. |
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| Authors: | ZHANG Hualin CHEN Zhiqing LI Yongmei FENG Jia |
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| Institution: | ZHANG Hualin1,CHEN Zhiqing2,LI Yongmei1,FENG Jia1(1.College of Stomatology,Ningxia Medical University,Yinchuan 750004,China,2.West China College of Stomatology,Sichuan University,Chengdu 610041,China) |
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| Abstract: | Objective To prepare PLGA/HA composite scaffolds by electrospinning and characterize their cell compatibility. Methods PLGA/HA composite scaffolds were prepared by electrospinning.Subsequently,rat bone-marrow-derived mesenchymal stem cells were cultured,expanded and seeded on the PLGA/HA scaffolds.The attachment and proliferation of cells on the scaffolds were investigated by SEM,MTT assay. Results The composite scaffolds had a three-dimensional structure,which mimicked the structure of the natural extracel... |
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| Keywords: | PLGA HA Electrospinning Cell compatibility |
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