尼美舒利与阿霉素联合应用对骨肉瘤MG-63细胞增殖、凋亡及衰老的影响

目的:研究尼美舒利与阿霉素联合应用对骨肉瘤MG-63细胞的作用及机理。方法:MTT法检测细胞的增殖，衰老相关性β-半乳糖苷酶(SAβ-Gal)染色检测细胞衰老，流式细胞仪测定细胞周期和凋亡，透射电镜显示MG-63细胞的形态变化。结果:当阿霉素浓度固定在0.75 mg/L时，与低浓度尼美舒利(25-200 μmol/L)...

Effect of Combined Nimesulide and Adriamycin on Proliferation,Apoptosis,and Senescence in Human Osteosarcoma Cell Line MG-63

Objective: To investigate the inhibition and apoptosis-induction effect of combination of Nimesulide and adriamycin on osteosarcoma MG-63 cells.Methods: MG-63 cells were treated with different concentrations of Nimesulide and adriamycin.Cell growth was analyzed with MTT assay.Cell cycle and apoptosis were tested by flow cytometry.Expression of β-galactosidase,a senescence induced marker,was analyzed by the β-Gal assay.Cell cycle related proteins were tested by Western blot.Results: The combination of Nimesulide and adriamycin inhibited the growth of MG-63 cells more effectively than adriamycin and in a concentration-time dependent way.The combination of adriamycin and lower concentration of Nimesulide induced a dose-dependent increase in G2/M phase arrest and apoptosis;while the combination of adriamycin and higher Nimesulide induced a senescence-like G0/G1 phase arrest,along with up-regulated expression of bax and p21 and down-regulated expression of bcl-2,cyclin E,C-myc,and CDK2.Conclusion: Cell growth inhibition rate was significantly increased by combination of Nimesulide and adriamycin,which provided the theoretical basis for clinical combination application.