| 利用噬菌体展示技术筛选人源抗肝癌单链抗体 |
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| 引用本文： | 袁玉峰,张中林,刘志苏,覃海泉,孙江阳,何跃明,钱群. 利用噬菌体展示技术筛选人源抗肝癌单链抗体[J]. 武汉大学学报(医学版), 2011, 32(5): 688-691 |
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| 作者姓名： | 袁玉峰 张中林 刘志苏 覃海泉 孙江阳 何跃明 钱群 |
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| 作者单位： | 武汉大学中南医院肝胆胰外科 湖北武汉430071 |
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| 摘 要： | 目的:利用噬菌体展示技术,从Griffinl Library人源噬菌体单链抗体库中筛选出人源抗肝癌单链抗体.方法:扩增Griffinl Library人源噬菌体单链抗体库后,以甲胎蛋白(AFP)为包被抗原,用免疫试管法富集筛选人源抗肝癌单链抗体,用ELISA法鉴定获得的阳性克隆,对其基因进行测序.将淘选出的阳性单链抗体...
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| 关 键 词： | 单链抗体 噬菌体展示 甲胎蛋白 |
Screening of Human Single-Chain Fv Antibody Against Hepatocellular Carcinoma by Phage Display |
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| YUAN Yufeng,ZHANG Zhonglin,LIU Zhisu,QIN Haiquan,SUN Jiangyang,HE Yueming,QIAN Qun. Screening of Human Single-Chain Fv Antibody Against Hepatocellular Carcinoma by Phage Display[J]. Medical Journal of Wuhan University, 2011, 32(5): 688-691 |
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| Authors: | YUAN Yufeng ZHANG Zhonglin LIU Zhisu QIN Haiquan SUN Jiangyang HE Yueming QIAN Qun |
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| Affiliation: | YUAN Yufeng,ZHANG Zhonglin,LIU Zhisu,QIN Haiquan,SUN Jiangyang,HE Yueming,QIAN Qun Dept.of Hepatobiliary and Pancreatic Surgery,Zhongnan Hospital of Wuhan University,Wuhan 430071,China |
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| Abstract: | Objective:To screening out the human single-chain Fv(scFv) antibody from the Griffin1 Library against hepatocellular carcinoma by phage display technique.Methods:The Griffin1 Library was amplified first and then the AFP was used as antigen to screen the human scFv.The positive clone was identified with ELISA,then the sequence of scFv gene was detected before it was transfected into E.coli HB2151 by CaCl2 method to express scFv induced by IPTG,and its expression level was detected by SDS-PAGE.The scFv was pu... |
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| Keywords: | Single Chain Antibody Phage Display Alpha-Fetoprotein |
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