| 应用单细胞多重PCR进行性别鉴定 |
| |
| 引用本文： | 易萍,李力,姚宏,俞丽丽. 应用单细胞多重PCR进行性别鉴定[J]. 中国优生与遗传杂志, 2005, 13(5): 23-24 |
| |
| 作者姓名： | 易萍 李力 姚宏 俞丽丽 |
| |
| 作者单位： | 第三军医大学大坪医院野战外科研究所妇产科,重庆,400042 |
| |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| |
| 摘 要： | 目的建立一种准确、快速、简便的在单细胞水平进行性别鉴定的技术.方法应用多重 PCR同时扩增单个活检细胞的Y染色体特异重复序列(DYZ1)及X染色体特异重复序列(DXZ1).结果 40个淋巴细胞(XY)成功地扩增出DXZ1电泳带(316bp)和DYZ1电泳带(154bp),正确率为100%;40个淋巴细胞(XX)中有39个成功地扩增出一条DXZ1电泳带(316bp),正确率为98%.结论采用DYZ1和DXZ1引物对单个细胞同时扩增进行性别鉴定是一种简单可靠的方法,可用于胚胎种植前诊断及无创性产前诊断.
|
| 关 键 词： | 性别鉴定 多重PCR |
| 文章编号： | 1006-9534(2005)-05-0023-02 |
| 修稿时间： | 2004-09-05 |
Multiplex polymerase chain reaction assay for sex determination from a single cell |
| |
| YI Ping,LI Li,YAO Hong,YU Li-li. Multiplex polymerase chain reaction assay for sex determination from a single cell[J]. Chinese Journal of Birth Health & Heredity, 2005, 13(5): 23-24 |
| |
| Authors: | YI Ping LI Li YAO Hong YU Li-li |
| |
| Abstract: | Objetive:To develop a accurate and rapid and simple method to determine sex from a single cell. Methods:We developed a multiplex polymerase chain reaction assay from a single lymphocyte to co-amplify X(DXZ1)-and Y(DYZ1)-specific repeat sequences,yields a 316 bp band in females and two bands of 154 and 316 bp in males.Results:All 40 of the XY cells were amplified the DXZ1 and DYZ1 bands successfully and diagnosed as male(100%),where as 39 of the 40 XX cells were amplified the DXZ1 band and diagnosed as female(97.5%).Conclusion:This method could be used in preimplantation genetic diagnosis and noninvasive prenatal diagnosis. |
| |
| Keywords: | DXZ1 DYZ1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
