| 血小板活化因子对幼鼠肠道免疫屏障功能的影响 |
| |
| 引用本文： | 王丽杰,刘冬妍,孙梅,赵恂. 血小板活化因子对幼鼠肠道免疫屏障功能的影响[J]. 世界华人消化杂志, 2005, 13(18): 2266-2268 |
| |
| 作者姓名： | 王丽杰 刘冬妍 孙梅 赵恂 |
| |
| 作者单位： | 中国医科大学附属第二临床学院儿科,辽宁省,沈阳市,110004;中国医科大学附属第二临床学院检验科,辽宁省,沈阳市,110004;中国医科大学实验技术中心二部,辽宁省,沈阳市,110001 |
| |
| 摘 要： | 目的:探讨血小板活化因子(plateletactivatingfactor,PAF)对肠黏膜分泌型IgA(secretoryIgA,SIgA)的影响.方法:用50μg/kg和65μg/kgPAF对大鼠进行腹腔注射(1μL/g),不同时间点处死动物,应用双抗体-PEG放射免疫法测定肠黏膜中SIgA含量,常规苏木精-伊红染色,光镜观察形态学改变.结果:PAF65组回肠0.5,1.5,3h可见绒毛水肿,固有层血管充血,间质淋巴管扩张,肠腔炎性渗出,上皮脱落,6,24h绒毛水肿.PAF50组0.5,1.5h可见绒毛水肿,固有层血管充血,3,6,24h绒毛水肿.实验组0.5,1.5,3,6hSIgA均较对照组显著降低(PAF50组分别为0.31±0.03mg/L,0.40±0.10mg/L,P<0.01,0.43±0.13mg/L,0.46±0.11mg/L,P<0.05;PAF65组分别为0.28±0.07mg/L,0.36±0.08mg/L,P<0.01,0.40±0.11mg/L,0.42±0.06mg/L,P<0.05vs0.66±0.10mg/L).0.5h下降幅度最大,随时间推移有逐渐升高趋势.结论:PAF可损害肠黏膜的免疫屏障功能,使SIgA降低.
|
| 关 键 词： | 血小板活化因子 肠道免疫屏障 幼鼠 |
| 修稿时间： | 2005-08-03 |
Effect of platelet activating factor on function of intestinal immunological barrier in young rats |
| |
| Li-Jie Wang,Dong-Yan Liu,Mei Sun,Xun Zhao. Effect of platelet activating factor on function of intestinal immunological barrier in young rats[J]. World Chinese Journal of Digestology, 2005, 13(18): 2266-2268 |
| |
| Authors: | Li-Jie Wang Dong-Yan Liu Mei Sun Xun Zhao |
| |
| Affiliation: | Li-Jie Wang,Mei Sun,Department of Pediatrics,the Second Aff iliated Clinical College,China Medical University,Shenyang 110004,Liaoning Province,China Dong-Yan Liu,Department of Medical Examination,the Second Affiliated Clinical College,China Medical University,Shenyang 110004,Liaoning Province,ChinaXun Zhao,the Second Department of Experimental Technique Center,China Medical University,Shenyang 110001,Liaoning Province,China |
| |
| Abstract: | |
| |
| Keywords: | Platelet activating factor Intestinal immunological barrier Young rats |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《世界华人消化杂志》浏览原始摘要信息 |
|
点击此处可从《世界华人消化杂志》下载全文 |
|
