胃癌体外药敏试验与化疗疗效的相关性研究

[目的]研究胃癌体外药物敏感性与临床疗效的相关性,为临床肿瘤个体化化疗提供依据.[方法]78例胃癌分为四甲基偶氮唑蓝(MTT)试验组和对照组.接受MTT药敏实验者,根据药敏结果分为敏感组和不敏感组,敏感组根据药敏结果指导化疗,不敏感组和对照组采用常规化疗方案化疗,观察各组临床近期疗效和3年生存率.[结果]47例胃癌组织对7种化疗药物的体外敏感性顺序依次为:羟基喜树碱、丝裂霉素、柔红霉素、斑蝥酸钠、氟尿嘧啶、顺铂、阿霉素,与临床实践不完全一致.MTT敏感组、不敏感组与对照组近期临床有效率分别为:69.2%(27/39),0(0/6),50.0%(14/28),差异有显著性(P＜0.05).MTT敏感组、不敏感组与对照组的3年生存率分别为59.5%(22/37),0(0/6),39.3%(11/28),有显著性差异(P＜0.05).[结论]MTT药敏试验结果有助于临床的个体化治疗和化疗效果的预测,有助于提高胃癌患者的远期生存率.

The Correlation Between Chemosensitivity in Vitro and Efficacy of Chemotherapy in Patients with Gastric

[Purpose]To study the correlation between chemosensitivity in vitro and efficacy of chemotherapy in patients with gastric cancer, and provide data for individualized chemotherapy. [Method] Se ventyeight patients with gastric cancer were divided into two groups: methyl thiazoly tetrazolium(MTT) group and control group. MTT group was divided into sensitive group and resistive group by degree of chemosensitivity in vitro. MTT sensitive group were treated according to the result of MTT assay. MTT resistive group and control group were treated by conventional chemotherapy. Shortterm response and 3year survival in all patients were analysed. [Result] The chemosensitivity order with drugs in 47 cases was hydroxycamptothecine, mitomycin, daunorubicin, sodium cantharidate, 5fluorouracil, cisplatin, adriamycin, and which were not consistent with conventional clinical practice. The shortterm response in MTT sensitive group was 69.2%(27/39); MTT resistive group, 0(0/6); and the control group, 50%(14/28)(P<0.05). And the 3year in MTT sensitive group was 59.5%(22/37); MTT resisive group, 0(0/6); and the control group, 39.3%(11/28)(P<0.05). [Conclusion] The result of MTT assay in vitro is help for individualized chemotherapy and prediction of efficacy, and improving the longterm survival in patients with gastric cancer.