Response of Müller cells following experimental lensectomy-vitrectomy |
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Authors: | Yusuf K Durlu Sei-ichi Ishiguro Akiko Yoshida Takezo Mito Makio Tsuchiya Makoto Tamai |
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Institution: | (1) Department of Ophthalmology, Tohoku University School of Medicine, 1-1 Seiryo-machi, 980 Sendai, Miyagi, Japan |
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Abstract: | We used morphological, biochemical and immunohistochemical methods to assess the response of Müller cells after experimental
lensectomy-vitrectomy in rabbits. We observed widened intercellular spaces between the Müller cells and nerve fibers of ganglion
cells, and increased electron opacity in the Müller cells of eyes injected with silicone oil. No apparent morphological changes
were detected in the Müller cells of air-injected eyes. The specific and total activities of Müller cell-marker enzymes (glucose
6-phosphatase and glutamine synthetase) showed an initial increase, followed by a decrease. Glial fibrillary acidic protein
immunoreactivity was not found in the Müller cells of the normal rabbit retina but was exhibited after surgery. Our results
showed that markers of Müller cells associated with glycogenolysis and/or gluconeogenesis, glutamate-glutamine cycle and cytoskeletal
protein metabolism were affected by the experimental lensectomy-vitrectomy. |
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