| T.th DNA聚合酶在检测登革病毒感染中的应用 |
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| 引用本文： | 姚海军. T.th DNA聚合酶在检测登革病毒感染中的应用[J]. 临床检验杂志, 1999, 0(2): 89-90 |
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| 作者姓名： | 姚海军 |
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| 作者单位： | 广州市卫生防疫站病毒免疫室，中山医科大学微生物学教研室 |
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| 摘 要： | 用T.th DNA聚合酶进行逆转录/多聚酶链反应(一步法)检测登革病毒4型(DV4)感染，通过与传统的逆转录/多聚酶链反应二步法比较，发现一步法敏感性是2个TCID50，二步法敏感性是3个TCID 50。对56例DV4患者急性期血清进行检测，一步法阳性率85.7%，二步法阳性率76.8%。
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| 关 键 词： | 登革病毒；逆转录／多聚酶链反应 |
Application of T.th DNA polymerase for the detection of Dengue 4 virus infection |
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| YAO Haijun,DUAN Fuqin,LIU Shuguo,et al.. Application of T.th DNA polymerase for the detection of Dengue 4 virus infection[J]. Chinese Journal of Clinical Laboratory Science, 1999, 0(2): 89-90 |
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| Authors: | YAO Haijun DUAN Fuqin LIU Shuguo et al. |
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| Abstract: | RT-PCR(one stepmethod)was carried out using T.th DNA polymerase to detect Dengue 4 virusinfection.Compared with traditional RT-PCR two step method,the sensitivity of one stepmethod is 2 TCID 50,the sensitivity of two step method is 3 TCID 50.56 sera from patientsinfected with Dengue 4 virus were detected using these two methods,the positive rate ofone step method and two step method are 85.7% and 76.8% respectively.One step method issensitive,specific,simple,rapid and economical,so it is applicable in laboratories. |
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