| 气相色谱法测定人体血中维生素E |
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| 引用本文： | 张伟利,陈瑞冠,任安林.气相色谱法测定人体血中维生素E[J].营养学报,1984(4). |
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| 作者姓名： | 张伟利 陈瑞冠 任安林 |
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| 作者单位： | 上海第二医学院附属新华医院儿内科
(张伟利，陈瑞冠)，上海医药工业研究院(任安林) |
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| 摘 要： | 作者用气相色谱法测定了人体血中α-生育酚的浓度。经过预处理去除血清中对α-生育酚测定有干扰的胆固醇后进行色谱分析。α-生育酚醇化为α-醋酸生育酚后保留时间约为8分钟,内标准采用三十四烷。本法回收率平均为76.4％,变异系数(CV)=4.4％(n=10)。25例脐血中α-生育酚测定值平均为2.1±0.5μg/ml,与分光光度法测定结果显著相关(r=0.6803,p<0.001)。
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THE ESTIMATION OF α-TOCOPHEROL IN HUMAN BLOOD BY GAS CHROMATOGRAPHY |
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| Abstract: | The estimation of a-tocopherol in human blood was carried out by gas chromatography. The cholesterol in plasma must he removed in advance. The a-tocopherol is chromatographed as tocopherol acetate and is eluted with a retention time of about 8 min. Tetratriacontane was used as internal standard. The average recovery rate was 76.4 per cent and coefficient of variation was 4.4 per cent (N = 10). The mean level in 25 cord blood was 2.1 ×0.5 μg/ml. It correlated well with the results by spectrophotome-tric method (r=0.6803 P<0.001). |
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