| 实时荧光定量PCR在流感病毒核酸检测中的应用 |
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| 引用本文： | 刘建荣,宋淑娥,何莹,姬莉莉,徐大鹏.实时荧光定量PCR在流感病毒核酸检测中的应用[J].职业与健康,2010,26(9):1000-1002. |
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| 作者姓名： | 刘建荣 宋淑娥 何莹 姬莉莉 徐大鹏 |
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| 作者单位： | 北京市怀柔区疾病预防控制中心,101400 |
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| 摘 要： | 目的通过对2009年6—11月北京怀柔地区流行性感冒(流感)病原学监测数据的分析,了解北京市怀柔区甲型H1N1型流感的流行趋势,为该地区甲型H1N1流感的防治提供科学依据。方法依据《全国流感监测实施方案》、《甲型H1N1流感病毒实验室检测技术方案》、《甲型H1N1流感监测方案(第二版)》、7500Fast型实时荧光定量PCR的SDS软件操作。结果2009年6—11月共采集流感样病例咽拭子1780件,检出流感病毒核酸阳性507件,阳性率为28.48%。阳性样本中,季节性甲3亚型92件,占18.15%;季节性甲1亚型8件,占1.58%;乙型4件,占0.79%;甲型H1N1型395件,占77.91%。结论2009年6—9月,甲型H1N1型流感的流行趋势为上升状态,但期间季节性甲3亚型仍为主要流行毒株。10—11月,甲型H1N1型流感的上升趋势达到高峰期,甲型H1N1流感病毒为流感流行主要毒株。
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| 关 键 词： | 流行性感冒 流感病毒 病原学监测 甲型H1N1 |
Application of Real-time PCR in Nucleic Acid Test of Influenza Virus |
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| LIU Jian-rong,SONG Shu-e,HE Ying,JI Li-li,XU Da-peng.Application of Real-time PCR in Nucleic Acid Test of Influenza Virus[J].Occupation and Health,2010,26(9):1000-1002. |
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| Authors: | LIU Jian-rong SONG Shu-e HE Ying JI Li-li XU Da-peng |
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| Institution: | LIU Jian-rong,SONG Shu-e,HE Ying,JI Li-li,XU Da-peng(Huairou District Center for Disease Control , Prevention,Beijing,101400,China) |
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| Abstract: | Objective]Through analysis on pathogen monitoring data of influenza virus in Huairou District of Beijing during June to November in 2009,to analyze the epidemic trend of Influenza A H1N1,and provide scientific evidence for control of it.Methods]The analysis was based on Implementation of National Influenza Surveillance Program,Laboratory Testing Technology solutions for Influenza A H1N1 Influenza Virus,Influenza A H1N1 Surveillance Program(2nd Edition) and the SDS software of 7500 Fast real-time fluoresce... |
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| Keywords: | Influenza Influenza virus Pathogen monitoring Influenza A H1N1 |
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