Validation of marrow fat assessment using noninvasive imaging with histologic examination of human bone samples |
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| Affiliation: | 1. Department of Therapeutic Radiology, University of Minnesota, Minneapolis, MN, USA;2. Osaka University, Osaka, Japan;3. Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA;4. Center for Magnetic Resonance Imaging, University of Minnesota, Minneapolis, MN, USA;5. Department of Medicine, University of Minnesota, Minneapolis, MN, USA;1. Center for Musculoskeletal Research, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, NY 14642, United States;2. Department of Biomedical Engineering, University of Rochester, 207 Robert B. Goergen Hall, Rochester, NY 14642, United States;3. Department of Orthopaedics, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, United States;1. Department of Health and Care Sciences, UiT–The Arctic University of Norway, Tromsø, Norway;2. Department of Clinical Medicine, UiT–The Arctic University of Norway, Tromsø, Norway;3. Department of Internal Medicine, University Hospital of North Norway, Tromsø, Norway;4. Department of Community Medicine, UiT, The Arctic University of Norway, Tromsø, Norway;5. Department of Clinical Endocrinology, Oslo University Hospital, Oslo, Norway;1. Division of Orthopedic Surgery, Department of Surgery, Children''s Hospital of Philadelphia, Philadelphia, PA, USA;2. Department of Medical Genetics and Skeletal Rare Diseases, IRCCS Rizzoli Orthopaedic Institute (IOR), Bologna, Italy;3. Center for Applied Genomics, Division of Human Genetics, Children''s Hospital of Philadelphia, Philadelphia, PA, USA;4. Department of Pathology & Laboratory Medicine, Children''s Hospital of Philadelphia, Philadelphia, PA, USA;5. Institute for Maternal and Child Health, IRCCS “Burlo Garofolo”, Trieste, Italy;6. Department of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy;7. Department of Pediatrics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA;8. Department of Pediatrics, Division of Pediatric Research, The University of Texas Medical School at Houston, Houston, TX USA;1. Centre for Physical Activity and Nutrition Research, School of Exercise and Nutrition Sciences, Deakin University, Melbourne, Australia;2. Griffith Health Institute, Centre for Musculoskeletal Research, School of Allied Health Sciences, Griffith University, Gold Coast, Australia;1. Center for Musculoskeletal Research, Department of Orthopaedics & Rehabilitation, University of Rochester Medical Center, Rochester, NY 14642, United States;2. Department of Biomedical Engineering, University of Rochester, Rochester, NY 14627, United States;3. Department of Orthopaedic Surgery, Washington University in St. Louis School of Medicine, St. Louis, MO 63110, United States;1. Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA;2. Department of Oral Biology, School of Dentistry, University of Missouri, Kansas City, MO 64110, USA |
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| Abstract: | PurposeThe marrow composition throughout the body is heterogeneous and changes with age. Due to heterogeneity, invasive biopsies of the iliac crest do not truly represent the complete physiological status, impeding the clinical effectiveness of this method. Therefore, we aim to provide verification for an in vivo imaging technique using co-registered histologic examinations for assessment of marrow adiposity.MethodsFive recently expired (i.e. < 24 h) human cadavers were scanned with a dual source CT (DECT) scanner in order to measure marrow fat in the lumbar vertebrae. These donors were also imaged using water–fat MRI (wfMRI) which was used to estimate the fraction of yellow marrow. After imaging, lumbar columns were excised and the superior and inferior aspects of 21 vertebrae were removed. The remaining center section was processed for histological examination to find the ratio of adipocyte volume per tissue volume (AV/TV).ResultsResults of DECT and wfMRI had a high correlation (r = 0.88). AV/TV ranged from 0.18 to 0.75 with a mean (SD) of 0.36 (0.18). Inter-evaluator reliability for AV/TV was r > 0.984. There were similar correlations between AV/TV and the imaging modalities, DECT-derived MF and wfMRI (r = 0.802 and 0.772, respectively).ConclusionsA high MF variation was seen among the 25 vertebrae imaged. Both DECT and wfMRI have a good correlation with the histologic adipocyte proportion and can be used to measure MF. This makes longitudinal studies possible without painful, less-effective, invasive biopsies. |
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