miR-34a-5p对HaCaT细胞增殖及其靶基因Notch1表达的影响

目的: 检测miR-34a-5p对人角质形成细胞株HaCaT细胞体外增殖及其靶基因Notch1 mRNA表达的影响。方法: HaCaT细胞体外培养，优化转染浓度,分为4组进行转染：miR-34a-5p 模拟物组（minic 组），阴性对照组（NC组），miR-34a-5p 抑制物组（inhibitor组），抑制物阴性对照组（inhibitor NC组）。MTT检测细胞增殖情况； RT-qPCR检测Notch1的mRNA表达情况。结果: 转染后48小时minic 组、inhibitor组细胞增殖活力分别为39.7%和15.7%，minic 组、inhibitor组与NC组（21.3%）相比，差异有统计学意义(Ps<0.05)。HaCaT细胞转染后，minic 组和inhibitor组Notch1表达量分别为2.78±1.30和0.37±0.46，与NC组(1.0±0.06)比较，差异均有统计学意义(P<0.05)。结论: miR-34a-5p促进HaCaT细胞体外增殖，机制可能与Notch1 mRNA表达上调有关。

Effects of miR-34a-5p on the proliferation and expressions of Notch1 in HaCaT Cells

Objective: To determine the effects of miR-34a-5p on proliferation of HaCaT human keratinocytes and expressions of Notch1 mRNA. Methods: HaCaT cells were cultured in vitro and transfection concentration were optimized. And than, the cells were divided into 4 groups: miR-34a-5p minic group (minic), negative control group (NC)，miR-34a-5p inhibitor group (inhibitor) and inhibitor negative control group (inhibitor NC). Cell proliferation was detected by MTT assay. The expression of Notch1 mRNA was detected by RT-qPCR. Results: After transfected for 48 hours, the proliferation vitality of HaCaT cells and the level of Notch1 in NC group were 20.7% and 1.0 ± 0.06 respectively, those in minic group were 39.7% and 2.78 ± 1.30, those in inhibitor group was 15.7% and 0.37 ± 0.46. There were significant differences in the proliferation vitality of HaCaT cells and the level of Notch1 between minic group, inhibitor group and NC group (Ps＜0.05). Conclusion: miR-34a can promote the proliferation of HaCaT cells, which may be related to the upregulation of Notch1 mRNA.