mTORC1信号通路在狼疮鼠体内的表达及意义

目的：检测mTORC1信号通路在系统性红斑狼疮鼠体内的表达，探讨其在系统性红斑狼疮发病中的作用。方法： 研究对象为6只B6.MRL/lpr狼疮小鼠和6只C57正常小鼠。RT-PCR和Western blot检测肾脏组织Akt、mTOR、p70S6K、IL-17 mRNA和蛋白的表达。细胞内染色流式细胞术检测脾脏Th17淋巴细胞亚群比例。结果： 狼疮鼠组Akt、mTOR、p70S6K、IL-17 mRNA、磷酸化蛋白表达水平高于对照组（P<0.05）；狼疮鼠组的Th17细胞占CD4+T细胞的比例高于正常对照组（P<0.05）。结论： mTORC1（Akt-mTOR-p70S6K）信号通路在SLE的发病中起着重要作用。

The expression of mTORC1 pathway in murine model of systemic lupus erythematosus

Objective: To detect the level of mTORC1 pathway related factors in the murine model of systemic lupus erythematosus (SLE) and to explore its effect in the development of SLE. Methods: There were 6 B6.MRL/lpr lupus mice in the experimental group and 6 C57 mice in the control group. The levels of Akt, mTOR, p70S6K, IL-17 mRNA and protein in kidney were detected by RT-PCR and western blot. The percentage of Th17 lymphocytes in CD4+T cells in spleen was detected by flow cytometry. Results: The mRNA and phosphoprotein of Akt, mTOR, p70S6K and IL-17 in the experimental group were higher than those in the control group. The percentage of Th17 lymphocytes in CD4+T cells in the experimental group was higher than that in the control group. Conclusion: The activation of mTORC1(Akt-mTOR-p70S6K) pathway may play an important role in the development of SLE.