| 腺嘌呤肾虚多尿大鼠肾脏CYP11B2 mRNA表达变化的研究 |
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| 引用本文： | 李淑雯,吴清和,黄 萍,操红缨.腺嘌呤肾虚多尿大鼠肾脏CYP11B2 mRNA表达变化的研究[J].中国实验方剂学杂志,2010,16(2):64-67. |
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| 作者姓名： | 李淑雯 吴清和 黄 萍 操红缨 |
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| 作者单位： | 1. 江西中医药高等专科学校,江西抚州,334000
2. 广州中医药大学中药学院,广东广州,510006 |
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| 基金项目： | 国家自然科学基金(30873425) |
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| 摘 要： | 目的:通过研究腺嘌呤对醛固酮合成酶途径的影响,探讨其导致"肾虚多尿"的机制所在。方法:利用酶联免疫分析法分别检测正常组、腺嘌呤组动物血中皮质酮(Cort)、醛固酮(ALD)的含量;并采用RT-PCR的方法检测各组动物肾脏CYP11B2 mRNA的表达。结果:与正常组比较,腺嘌呤可明显减少大鼠血中Cort,ALD含量,并下调模型大鼠肾脏CYP11B2mRNA的表达。结论:腺嘌呤肾虚多尿大鼠血中Cort,ALD含量减少,肾脏CYP11B2 mRNA的表达下调,影响醛固酮合成酶途径,从而减少ALD的合成,导致水液代谢异常引发多尿,本实验研究结果从醛固酮合成酶的角度阐明了腺嘌呤导致"肾虚多尿"的作用机制。
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| 关 键 词： | 腺嘌呤 肾虚多尿 醛固酮合成酶 |
| 收稿时间： | 2009/9/11 0:00:00 |
The Experimental Study on the mRNA Expression of CYP11B2 in Rat with Kidney Deficiency and Diuresis Induced by Adenine |
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| LI Shu-wen,WU Qing-he,HUANG Ping and CAO Hong-ying.The Experimental Study on the mRNA Expression of CYP11B2 in Rat with Kidney Deficiency and Diuresis Induced by Adenine[J].China Journal of Experimental Traditional Medical Formulae,2010,16(2):64-67. |
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| Authors: | LI Shu-wen WU Qing-he HUANG Ping and CAO Hong-ying |
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| Institution: | Jiangxi College of Traditional Chinese Medicine, Fuzhou 344000,China;College of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine,Guangzhou 510006,China;College of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine,Guangzhou 510006,China;College of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine,Guangzhou 510006,China |
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| Abstract: | Objective:To explore the mechanism for kidney deficiency and diuresis by studying the action of adenine to ALD synthase.Methods:The contents of Cort,ALD in blood were determined and the mRNA expression of CYP11B2 was evaluated with ELISA and RT-PCR in model group and control group.Results:Compared with control group,adenine could remarkedly decrease the contents of Cort,ALD in rat blood;and downregulate the mRNA expression of CYP11B2.Conclusion:Adenine can decrease the contents of Cort,ALD in blood and down... |
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| Keywords: | adenine kidney deficiency and diuresis ALD synthase |
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