Intrinsic expression of Nod2 in CD4+ T lymphocytes is not necessary for the development of cell-mediated immunity and host resistance to Toxoplasma gondii |
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Authors: | Caetano Braulia C Biswas Amlan Lima Djalma S Benevides Luciana Mineo Tiago W P Horta Catarina V Lee Kyoung-Hee Silva Joao S Gazzinelli Ricardo T Zamboni Dario S Kobayashi Koichi S |
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Institution: | Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA. |
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Abstract: | Nod2 belongs to the nucleotide-binding domain leucine-rich repeat family of proteins and senses bacterial cell wall components to initiate innate immune responses against various pathogens. Recently, it has been reported that T-cell-intrinsic expression of Nod2 promotes host defense against Toxoplasma gondii infection by inducing type 1 immunity. Here, we present results that demonstrate that Nod2 does not play a role in the defense against T. gondii infection. Nod2-deficient mice were fully capable of inducing Th1 immune responses and did not show enhanced susceptibility to infection. Upon TCR stimulation in vitro, Nod2-deficient CD4(+) T cells showed normal activation, IL-2 production, proliferation, and Th1/2 differentiation. Nod2 mRNA and protein were expressed in CD4(+) T and CD8(+) T cells at substantial levels. Therefore, Nod2, although expressed in CD4(+) T cells, does not have an intrinsic function in T-cell activation and differentiation. |
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Keywords: | Nod2 Nucleotide‐binding domain leucine‐rich repeat proteins (NLR) Toxoplasma gondii |
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