GAD—Ab、ICA检测在糖尿病分型诊断中的临床价值

目的探讨血清谷氨酸脱羧酶抗体（GAD-Ab）、胰岛细胞抗体（ICA）联合检测对糖尿病分型的诊断价值。方法采用RIA法和ELISA法测定138例糖尿病患者及38例正常人的血清GAD—Ab、ICA水平。结果1型糖尿病患者GAD．Ab、ICA阳性率分别为57．1％和38．8％，联合检测阳性率为91．8％，与其他组比较差异有统计学意义（P〈0．01）；2型糖尿病患者GAD-Ab、ICA阳性率分别为9．0％和19．1％，与对照组比较差异有统计学意义（P〈0．05）。GAD-Ab和ICA抗体阳性患者C肽水平较低。结论GAD-Ab、ICA可用于糖尿病的分型诊断，联合检测能提高阳性率，且有助于检出成人隐匿性免疫糖尿病（LADA）。

Clinical Value of Serum Glutamic Acid Decarboxylase Antibody and Islet Cell Antibody Test in Typing Diagnosis of Diabetes Mellitus

Objective To evaluate the diagnostic values of combining detection of serum glutamie acid decarboxylase antibody （GAD-Ab） and islet cell antibody（ICA.） in diabetes typing. Methods The blood samples of 138 patients diagnosed clinically as diabetes mellitus and 38 health individuals were taken to measure GAD-Ab and ICA by the methods of RIA arid ELISA respectively. Results The positive rate of GAD-Ab and ICA in the patients with type 1 diabetes was 57.1% and 38.8% respectively, joint testing positive rate was 91.8%, and there were significant difference compared to other groups（ P 〈 0.01 ）. The positive rate of GAD-Ab and ICA in the patients with type2 diabetes was 9.0% and 19.1% ,and there was significant difference compared to control group（P 〈0.05）. The serum C peptide levels in the patients with positive antibodies was lower than that of those with negative antibodies. Conclusions GAD-Ab and ICA can be used to the typing diagnosis of diabetes,and the joint detection can improve the positive rate and help to detect latent autoimmune diabetes adults（LADA）.