丙型肝炎病毒核心抗原检测试剂盒的临床应用评价

目的采用酶联免疫吸附试验（ELISA）检测丙型肝炎病毒核心抗原（HCVcAg）。方法对苏州市吴中人民医院2011年2月至2012年2月的2 523份输血、手术前住院患者血清标本进行抗-HCV初、复检检测。将初、复检均阳性的其中10份、仅初检阳性的15份和仅复检阳性的17份血清标本分别采用ELISA检测HCVcAg和采用RT聚合酶链反应（RT-PCR）检测HCV。结果 ELISA检测HCVcAg阳性结果为4份（40%）。32份仅初检或复检抗-HCV阳性标本采用ELISA检测HCVcAg阳性6份,阳性率为18.75%。采用RT-PCR荧光定量检测HCV 10份初、复检抗-HCV均阳性的血清标本结果均为阳性,32份仅初检或复检抗-HCV阳性标本采用RT-PCR荧光定量检测HCV阳性5份,阳性率为15.63%。结论 HCVcAg的敏感性与RT-PCR荧光定量检测HCV类似,能对HCV的感染作出早期诊断。

Clinical detection of hepatitis C core antigen ELISA reagent

Objective To detect hepatitis C virus core antigen（HCVcAg） by enzyme-linked immunosorbent assay（ELISA）.Methods 2523 patients′ serum samples were selected from before blood transfusion and operation for anti-HCV early re-verification tests from February 2011 to February 2012.10 samples with being both positive in first test and retest,15 samples with being positive in first test and 17 samples with being positive in retest were detected by ELISA for HCVcAg and RT-PCR fluorescence quantitative detection for HCV.Results Four（4/10）were positive by ELISA.32 sample with initial inspection or re-examination both positive of anti-HCV positive were tested by ELISA HCV the-cAg,the positive rate was 18.75%.10 samples with initial inspection or re-examination both positive of HCV were positive by RT-PCR fluorescence quantitative,32 sample with initial inspection or re-examination both positive of anti-HCV positive were tested by RT-PCR,positive rate was 15.63%.Conclusion Of HCVcAg has sensitivity as testing HCV by RT-PCR fluorescence quantitative,it can make an early diagnosis of HCV infection.