地氟烷对全脑缺血再灌注损伤大鼠脑超微结构的影响

目的研究新型吸入麻醉药地氟烷对全脑缺血再灌注损伤大鼠脑超微结构的影响,探讨其脑保护作用。方法9只W istar大鼠分为3组,缺血组(n=3)、地氟烷组(n=3)和假手术对照组(n=3)。制备大鼠全脑缺血再灌注模型。地氟烷组再灌注开始后立即吸入地氟烷1 h。缺血组和地氟烷组于缺血再灌注1 h、假手术组于手术后1 h取脑,利用电镜技术观察皮质超微结构的变化。结果与缺血组比较,地氟烷组神经元固缩少,神经元的细胞器和微管结构基本正常,但胶质细胞和微循环的破坏并无改善。结论地氟烷对神经元、细胞骨架可能具有保护作用,但对胶质细胞和微循环可能无保护作用。

Effects of Desflurane on Ultrastructure in Rats with Reperfusion Injury in Global Cerebral Ischemia

Objective To observe the effect of desflurane on ultrastructure in rats with global cerebral ischemia reperfusion injury and investigate the cerebral protection action of deflurane.Methods Nine Wistar rats were randomly divided into 3 groups: ischemia group(n=3),desflurane group(n=3),and sham-operation group(n=3).Rat models with global cerebral ischemia-reperfusion were prepared.The desflurane inhalation for one hour was given immediately to the desflurane group when the reperfusion started.The brain was obtained 1 h after the ischemia-reperfusion in the ischemia and desflurane groups and after the operation in the sham-operation group.The changes of cortical ultrastructure were observed with electron microscope.Results The pyknosis of neurons in the desflurane group was less,and the organelle and microtubule-structure were basically normal,but the gliocytes and microcirculation were not improved,as compared with those of the ischemia group.Conclusion The results indicated that desflurane may have protective effects on neurons and cytoskeletons,but not on the gliocytes and microcirculations.