奥沙利铂联合吉西他滨对肝癌细胞株HEPG-2生长抑制作用的研究

目的：通过对奥沙利铂（L-OHP）、吉西他滨（GEM）单独及联合作用抑制肝癌细胞株HepG2生长的实验研究,探讨两药合用的效果及最佳剂量。方法：采用一定浓度梯度L-OHP和GEM单独及联合与人肝癌细胞株HepG2孵育后,采用MTT法检测细胞生长变化,并采用两药相互作用系数来评价两药相互作用性质。结果：在各作用时间点,联合组对肝癌细胞HepG2的生长抑制率与相应浓度L-OHP、GEM单药组相比均显著增强。结论：L-OHP与GEM联用可增强互补,减少各自用药剂量,达到减毒增效的目的。

A study on the repression of oxaliplatin combined with gemcitabine to hepatocellular carcinoma cell line HepG2

Objective： To observe the cooperative effect and the optimal dose of oxaliplatin（L-OHP）,gemcitabine（GEM） singly or combined on hepatocellular carcinoma cell line HepG2.Methods： The human hepatocellular carcinoma cell line HepG2 was cultured with L-OHP combined with/without GEM in certain concentration gradient.MTT was used to observe the cell growth inhibition.The character of the interaction between two drugs was estimated by coefficient of drug in interaction（CDI）.Results： The effect of their combination was higher than either of them alone in inhibiting growth of HepG2（P0.01）.Furthermore,the combination of these two drugs had a synergistic effect.Conclusion： The combination of L-OHP and GEM can increase the effect against hepatoceullar carcinoma and reduce their dosage and toxicity.