| 胎牛血清促进色素上皮衍生因子在皮肤角质形成细胞和成纤维细胞中的表达 |
| |
| 引用本文： | 满孝勇,姚永刚,杨晓红,蔡绥勍,郑敏. 胎牛血清促进色素上皮衍生因子在皮肤角质形成细胞和成纤维细胞中的表达[J]. 浙江大学学报(医学版), 2009, 38(4). DOI: 10.3785/j.issn.1008-9292.2009.04.003 |
| |
| 作者姓名： | 满孝勇 姚永刚 杨晓红 蔡绥勍 郑敏 |
| |
| 作者单位： | 1. 浙江大学医学院,附属第二医院皮肤科,浙江,杭州,310009
2. 中国科学院昆明动物研究所遗传与疾病重点实验室,云南,昆明,650223 |
| |
| 基金项目： | 国家自然科学基金，浙江省卫生厅资助项目 |
| |
| 摘 要： | 目的:探讨表皮角质形成细胞和真皮成纤维细胞中色素上皮衍生因子(PEDF) 表达的影响因素.方法:体外培养角质形成细胞和成纤维细胞,并以10%FBS(胎牛血清)刺激,通过免疫荧光和Western blot检测PEDF的表达.结果:PEDF大多位于细胞浆中,但在细胞核中也有少量表达.细胞浆中PEDF并非均质型分布,而是呈细颗粒状集聚.10%FBS促进角质形成细胞和成纤维细胞中PEDF的集聚和表达,而且这一分布形式不受组胺和佛波肉豆蔻醋酸(PMA) 的影响.结论:10% FBS促进角质形成细胞和成纤维细胞中PEDF的集聚和表达.
|
| 关 键 词： | 角蛋白细胞 成纤维细胞/细胞学 眼蛋白质类 真皮/细胞学 丝氨酸内肽酶类 细胞,培养的 |
Fetal bovine serum enhances expression of PEDF in epidermal keratinocytes and dermal fibroblasts |
| |
| MAN Xiao-yong,YAO Yong-gang,YANG Xiao-hong,CAI Sui-qing,ZHENG Min. Fetal bovine serum enhances expression of PEDF in epidermal keratinocytes and dermal fibroblasts[J]. Journal of Zhejiang University. Medical sciences, 2009, 38(4). DOI: 10.3785/j.issn.1008-9292.2009.04.003 |
| |
| Authors: | MAN Xiao-yong YAO Yong-gang YANG Xiao-hong CAI Sui-qing ZHENG Min |
| |
| Abstract: | Objective: To investigate the effect of fetal bovine serum (FBS) on expression of pigment epithelium-derived factor (PEDF) in normal epidermal keratinocytes and dermal fibroblasts. Methods: Keratinocytes and fibroblasts were incubated with 10% FBS. PEDF protein level in the cells was determined by immunofluorescence and Western blot. Results: PEDF was localized mostly in the cytoplasm,while some in the nuclei.The distribution of PEDF in cytoplasm was in a granular pattern.10% FBS increased the expression of PEDF both in keratinocytes and fibroblasts,but histamine and Phorbol 12-myristate 13-acetate (PMA) did not interfere the distribution of PEDF in cells. Conclusion: 10% FBS can upregulate expression of PEDF in epidermal keratinocytes and dermal fibroblasts. |
| |
| Keywords: | Keratinocytes Fibroblasts/cytol Eye proteins Dermis/cytol Serine endopeptidases Cells,cultured |
| 本文献已被 万方数据 等数据库收录！ |
|
