LINC01060对人胃癌BGC-823细胞系生物学行为的作用研究

目的:探索LINC01060对人胃癌BGC-823细胞系生物学行为的作用。方法:生信数据分析LINC01060在胃癌中的表达情况，建立LINC01060过表达和沉默的稳转BGC-823细胞株，Real time PCR验证LINC01060的表达，CCK-8检测细胞增殖，流式细胞术检测细胞凋亡，划痕实验检测细胞迁移，Transwell检测细胞侵袭，Western blot检测EMT相关蛋白E-cadherin、Vimentin、N-cadherin和Snail蛋白的表达。结果:GEO数据库发现，LINC01060在胃癌中低表达（P＜0.05）；Real time PCR结果显示，沉默组LINC01060的表达显著下调（P＜0.05），过表达组LINC01060的表达显著上调（P＜0.05）；CCK-8显示，过表达组细胞增殖能力显著下降（P＜0.05）；流式细胞术显示，过表达组细胞凋亡比例显著上升（P＜0.05）；Transwell结果显示，过表达组细胞侵袭能力显著下降（P＜0.05）；细胞划痕显示，过表达组细胞迁移能力显著下降（P＜0.05）；Western blot显示，过表达组E-cadherin蛋白表达水平显著上调（P＜0.05），Vimentin、N-cadherin、Snail蛋白表达水平显著下调（P＜0.05）；以上实验沉默组均相反。结论:LINC01060在人胃癌BGC-823细胞中发挥促进凋亡，抑制增殖、侵袭、迁移和EMT的作用。

The effect of LINC01060 on biological behavior of human gastric cancer BGC-823 cell line

Objective:To explore the effect of LINC01060 on biological behavior of human gastric cancer BGC-823 cell line.Methods:The expression of LINC01060 in gastric cancer was analyzed by GEO database,and the stable BGC-823 cell line with overexpression and silencing of LINC01060 was established.The expression of LINC01060 was verified by Real time PCR.Cell proliferation was detected by CCK-8 assay.Cell apoptosis was detected by flow cytometry.Cell metastasis was detected by scratch assay.Cell invasion was detected by Transwell assay,and the expressions of EMT-related proteins E-cadherin,Vimentin,N-cadherin and Snail were detected by Western blot.Results:The GEO database found that the expression of LINC01060 was low in gastric cancer (P＜0.05).Real time PCR results showed that the expression of LINC01060 in the silence group was significantly down-regulated (P＜0.05),and the expression of LINC01060 in the over-exposure group was significantly up-regulated (P＜0.05).CCK-8 showed that the proliferation ability of cells in the overexpression group was significantly decreased (P＜0.05).Flow cytometry showed that the proportion of cells apoptosis in the overexpression group was significantly increased (P＜0.05).Transwell results showed that the cell invasion ability of the overexpression group was significantly decreased (P＜0.05).Cell scratch showed that the cell migration ability of the overexpression group was significantly decreased (P＜0.05).Western blot showed that the expression level of E-cadherin protein in the overexpression group was significantly up-regulated (P＜0.05),and Vimentin,N-cadherin,Snail protein expression level was significantly down-regulated (P＜0.05).The above experiments were the opposite in silence group.Conclusion:LINC01060 can promote apoptosis and inhibit proliferation,invasion,migration and EMT in human gastric cancer BGC-823 cell line.