miR-181b-5P和EPB41L3蛋白在喉癌中的表达及临床意义

目的 miR-181b-5P和红细胞膜蛋白配体4.1样3(EPB41L3)目前在喉癌中表达关系及作用机制尚不明确。论文主要通过研究miR-181b-5P、EPB41L3蛋白在喉癌组织及癌旁正常组织中表达水平并进行相关性分析,探讨miR-181b-5P靶向调控EPB41L3对喉癌组织增殖、侵袭及转移的影响。 方法 通过生物信息学数据库预测miR-181b-5P在喉癌中的表达情况,采用实时荧光定量PCR技术测定喉癌及癌旁正常组织中miR-181b-5P的表达量。预测miR-181b-5p的靶基因并进行生物信息学分析,EPB41L3蛋白的相对表达由免疫组化SP法测定。 结果 喉癌组织中miR-181b-5P的表达明显较癌旁正常组织高(P<0.001),且在有无淋巴结转移间有显著差异(P=0.027 3)。EPB41L3蛋白在喉癌组织的表达明显较癌旁正常组织低(P<0.001)。EPB41L3蛋白在喉癌中的阳性表达率于不同病理分化程度、临床分期及有无淋巴结转移中差异具有统计学意义(P<0.05)。miR-181b-5p及EPB41L3蛋白在喉癌组织中的表达呈负相关(r=-0.420 8,P=0.002 3)。 结论 miR-181b-5P可能通过负性调控EPB41L3的表达,影响喉癌组织的增殖、侵袭、转移,研究可为喉癌的临床靶向治疗提供新的思路。

Expression and clinical significance of miR-181b-5P and EPB41L3 protein in laryngeal squamous cell carcinoma

Objective Currently, the expression relationship and mechanism of miR-181b-5P and Erythrocyte Membrane Protein Band 4.1-like 3(EPB41L3)in laryngeal cancer are still unclear. This paper mainly studied the expression levels of miR-181b-5P and EPB41L3 protein in laryngeal squamous cell carcinoma tissues and adjacent normal tissues, and than conducted correlation analysis. To explore the effects of miR-181b-5P targeted regulation of EPB41L3 on the proliferation, invasion and metastasis of laryngeal neoplasms. Methods Using bioinformatics databases predicted the expression of miR-181b-5P in laryngeal cancer, and quantitative real-time PCR was used to determine the expression of miR-181b-5P in laryngeal squamous cell carcinoma and adjacent normal tissues. The target genes of miR-181b-5P were predicted and analyzed by bioinformatics. The relative expression of EPB41L3 protein was detected by immunohistochemistry. Results The expression of miR-181b-5P in laryngeal carcinoma tissues was significantly higher than that in adjacent normal tissues(P<0.001), and there was significant difference in laryngeal carcinoma tissues with or without lymph node metastasis(P=0.027 3).The expression of EPB41L3 protein in laryngeal carcinoma tissues was significantly lower than that in adjacent normal tissues(P<0.001).The positive expression rate of EPB41L3 protein was obviously different in different pathological differentiation degrees, clinical stages and lymph node metastasis(P<0.05). The expressions of miR-181b-5p and EPB41L3 protein in laryngeal carcinoma tissues were negatively correlated(r=-0.420 8, P=0.002 3). Conclusion miR-181b-5p may negatively regulate the expression of EPB41L3 and than affect the proliferation, invasion and metastasis of laryngeal cancer. This study maybe provide new ideas for clinical targeted therapy of laryngeal cancer.