外周血单核细胞DNMT1及血清IL-6在糖尿病肾脏病中的表达及意义

目的 探讨外周血单核细胞DNA甲基化转移酶1(DNMT1)及血清白介素-6(IL-6)在糖尿病肾脏病（DKD）中的表达及临床意义。方法 选取150例DKD患者（DKD组）及50例同期健康体检者（对照组）。比较2组IL-6、DNMT1、总胆固醇、三酰甘油、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、血肌酐、空腹血糖、肾小球滤过率估计值（e GFR）、24 h尿白蛋白排泄率（24 h UAER）的差异。Pearson相关分析IL-6、DNMT1与其他临床指标的相关性。多因素Logistic回归分析DKD发生的影响因素。受试者工作特征（ROC）曲线分析24 h UAER、IL-6、DNMT1及联合检测对DKD的诊断价值。结果 与对照组比较，DKD组的空腹血糖、IL-6、DNMT1、血肌酐、24 h UAER、LDL-C、糖化血红蛋白水平升高，而eGFR、HDL-C水平降低（均P<0.05）。DKD组的IL-6与DNMT1呈正相关（r=0.560,P<0.05）；且IL-6、DNMT1与24 h UAER呈正相关（r分别为0.551和0.570,P<0...

Expression and significance of peripheral blood mononuclear cell DNMT1 and serum IL-6 in diabetic nephropathy

Objective To investigate the expression and clinical significance of peripheral blood mononuclear cell DNA methyltransferase 1 (DNMT1) and serum interleukin-6 (IL-6) in diabetic nephropathy (DKD). Methods A total of 150 DKD patients (the DKD group) and 50 healthy subjects (the control group) were selected. Data of IL-6, DNMT1, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), serum creatinine, fasting blood glucose, estimated glomerular filtration rate (eGFR), 24-hour urinary albumin excretion rate (24 h UAER) and other clinical indicators were compared between the two groups. Pearson correlation was used to analyze the correlation between IL-6, DNMT1 and other clinical indicators. Multivariate Logistic regression was used to analyze the influencing factors of DKD. Receiver operating curve (ROC) was used to analyze the diagnostic value of IL-6, DNMT1 and combined detection in DKD. Results Compared with the control group, levels of fasting blood glucose, IL-6, DNMT1, serum creatinine, 24 h UAER, LDL-C and glycosylated hemoglobin were higher in the DKD group, but levels of eGFR and HDL-C were lower (P<0.05). IL-6 was positively correlated with DNMT1 in the DKD group (r=0.560, P<0.05), and IL-6, DNMT1 and 24 h UAER were positively correlated (r=0.551, 0.570, P<0.05). Elevated 24 h UAER, elevated IL-6 and elevated DNMT1 were independent risk factors for DKD (P<0.05). The combined detection of 24 h UAER, IL-6 and DNMT1 in the area under the surface of DKD was significantly higher than that of single index detection. Conclusion DNMT1 and IL-6 are highly expressed in DKD patients, and the combined detection of 24 h UAER, DNMT1 and IL-6 can be used as a good indicator for clinical diagnosis of DKD.