M6A去甲基化酶FTO在脑胶质瘤中的表达及其与病人预后的关系

目的 探讨6-甲基腺嘌呤（m6A）去甲基化酶肥胖相关蛋白（FTO）在脑胶质瘤中的表达及其与病人生存预后的关系。方法 选取2015年1月至2017年8月手术切除的脑胶质瘤标本84例（WHOⅡ级24例，Ⅲ级14例，Ⅳ级46例）和颅脑损伤内减压术中获取的非肿瘤脑组织40例为对照。免疫印迹法和免疫组化法检测组织FTO表达水平。胶质瘤病人随访截止2022年8月，计算总生存期。结果 免疫组化染色显示，WHO分级Ⅱ、Ⅲ、Ⅳ级脑胶质瘤组织FTO高表达率分别为20.83%(5/24)、50.00%(7/14)、73.91%(34/46)，均明显高于非肿瘤脑组织10%(4/40);P<0.05]；而且，WHO分级Ⅳ级胶质瘤FTO过表达率明显高于Ⅱ级胶质瘤（P<0.05）。免疫印迹法检查结果显示脑胶质瘤组织FTO蛋白表达水平明显高于非肿瘤脑组织（P<0.05）。截止随访结束，脑胶质瘤死亡30例，其中高表达组死亡18例，低表达组死亡12例。多因素logistic回归分析显示，FTO高表达是胶质瘤预后不良的独立危险因素（OR=3.794;95%CI 1.164～5.108;P<0.00...

Expression of m6A demethylase FTO in glioma tissues and its relationship with patients' prognoses

Objective To investigate the expression of 6-methyladenine (m6A) demethylase obesity-related protein (FTO) in glioma tissues and its relationship with patients' survival prognoses. Methods The expression of FTO was detected in glioma tissues obtained from 84 glioma patients who underwent surgery from January 2015 to August 2017 (24 cases of WHO grade Ⅱ, 14 cases of WHO grade Ⅲ, 46 cases of Ⅳ) and in non-tumor brain tissue obtained from 40 patients with traumatic brain injury who underwent decompression by western blotting and immunohistochemistry, respectively. The glioma patients were followed up until August 2022, and the overall survival time was calculated. Results Immunohistochemical staining showed that the high expression rates of FTO in glioma tissue of WHO grade Ⅱ, Ⅲ, and Ⅳ were 20.83% (5/24), 50.00% (7/14) and 73.91% (34/46), respectively, which were significantly higher than that 10% (4/40)]in non-tumor brain tissue (P<0.05). In addition, the high expression rate of FTO in WHO grade Ⅳ gliomas was significantly higher than that in grade Ⅱ gliomas (P<0.05). Western blotting showed that the expression level of FTO protein in glioma tissues was significantly higher than that in non-tumor brain tissues (P<0.05). By the end of follow-up, 30 glioma patients died, including 18 patients with high expression of FTO and 12 with low expression. Multivariate logistic regression analysis showed that high expression of FTO was an independent risk factor for poor prognosis of glioma patients (OR=3.794; 95% CI 1.164~5.108; P<0.001). Survival curve analysis showed that the median overall survival time was significantly shorter in the patients with high FTO expression than the patients with low FTO expression (P<0.001). Conclusions The m6A demethylase FTO is highly expressed in glioma tissues, which is associated with a poor survival prognosis of glioma patients.