血清miR-149-3p和CXCR3水平与aSAH后迟发性脑缺血的相关性分析

目的 探讨血清微小RNA-149-3p(miR-149-3p)、CXC趋化因子受体3(CXCR3)与动脉瘤性蛛网膜下腔出血（aSAH）后迟发性脑缺血（DCI）的相关性。方法 2020年6月～2022年6月前瞻性收集aSAH共175例，采用qRT-PCR法检测血清miR-149-3p，采用酶联免疫吸附法检测血清CXCR3水平。结果 175例中，58例发生DCI，发生率为33.1%;117例未发生DCI。与未发生DCI病人比较，DCI病人血清miR-149-3p水平显著降低（P<0.05）,CXCR3水平显著升高（P<0.05）。aSAH病人血清miR-149-3p水平与CXCR3水平呈明显负相关（r=-0.462,P<0.001）；多因素logsitic回归分析显示，miR-149-3p水平降低、CXCR3水平增高是aSAH后发生DCI的独立危险因素（P<0.05）。ROC曲线分析显示，血清miR-149-3p水平≤0.94预测a SAH后发生DCI的曲线下面积（AUC）为0.828，敏感度为82.76%，特异度为72.65%；血清CXCR3水平≥12.81 ng...

Correlation of serum levels of miR-149-3p and CXCR3 with delayed cerebral ischemia after aneurysmal subarachnoid hemorrhage

Objective To investigate the correlation between serum levels of microRNA-149-3p (miR-149-3p) and CXC chemokine receptor 3 (CXCR3) and delayed cerebral ischemia (DCI) after aneurysmal subarachnoid hemorrhage (aSAH). Methods A total of 175 patients with aSAH were prospectively recruited from June 2020 to June 2022. Serum levels of miR-149-3p and CXCR3 were detected by qRT-PCR and enzyme-linked immunosorbent assay, respectively. Results Of these 175 patients, 58 patients had DCI (33.1%), and 117 did not. The incidence of DCI was 33.1%. Compared with patients without DCI, the serum level of miR-149-3p was significantly decreased (P<0.05) and the serum CXCR3 level was significantly increased (P<0.05) in patients with DCI. The serum level of miR-149-3p was significantly negatively correlated with the serum level of CXCR3 in aSAH patients (r=-0.462, P<0.001). Multivariate logsitic regression analysis showed that a decrease in serum level of miR-149-3p and an increase in serum level of CXCR3 were independent risk factors for DCI after aSAH (P<0.05). ROC curve analysis showed that the area under the curve (AUC) of serum miR-149-3p level ≤0.94 for predicting DCI after aSAH was 0.828, the sensitivity was 82.76%, and the specificity was 72.65%. The AUC of serum CXCR3 level ≥12.81 ng/L for predicting DCI after aSAH was 0.812, the sensitivity was 79.31%, and the specificity was 83.76%. The AUC of serum miR-149-3p ≤0.94 combined with serum CXCR3 ≥12.81 ng/L for predicting DCI after aSAH was 0.906, the sensitivity was 91.38%, and the specificity was 83.76%. The prediction effectiveness of two indexes was significantly better than that of a single index (P<0.05). Conclusions A decrease in serum level of miR-149-3p and an increase in serum level of CXCR3 are independent risk factors for DCI after aSAH, which may be used as indicators to predict DCI after aSAH.