| 2014—2018年仙桃市伤寒沙门菌分子分型及耐药性分析 |
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| 引用本文： | 叶恒平,张雅婷,袁敏,邢学森,吕静,何飞,周海健,李国明,杨红梅. 2014—2018年仙桃市伤寒沙门菌分子分型及耐药性分析[J]. 国际检验医学杂志, 2020, 0(9): 1042-1046,1051 |
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| 作者姓名： | 叶恒平 张雅婷 袁敏 邢学森 吕静 何飞 周海健 李国明 杨红梅 |
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| 作者单位： | 仙桃市疾病预防控制中心;湖北省疾病预防控制中心;中国疾病预防控制中心传染病预防控制所 |
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| 基金项目： | “十三五”国家科技重大专项子课题(2017ZX10103005003);湖北省卫生健康委员会科研项目(WJ2018H265、WJ2018H271)。 |
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| 摘 要： | 目的了解仙桃市伤寒沙门菌的耐药性及分子分型特征。方法收集2014—2018年分离的29株伤寒沙门菌开展药敏试验、脉冲场凝胶电泳(PFGE)分型及多位点序列分型(MLST)分析。结果药敏结果显示,29株伤寒沙门菌中27株菌对26种临床常见抗菌药物均为敏感/中度敏感,余2株菌有不同程度的耐药性,其中1株菌株耐药谱分布较广,对β-内酰胺/β-内酰胺抑制剂复合物类药物耐药。PFGE结果显示,29株伤寒沙门菌可分为6种PFGE型别,其中流行优势型为HuBXTSal003型别。MLST分型显示,29株菌株共有2种序列型,其中ST2为优势ST型。结论仙桃市伤寒沙门菌株存在优势PFGE带型及优势ST型,有多重耐药菌株出现。
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| 关 键 词： | 伤寒沙门菌 药敏试验 脉冲场凝胶电泳 多位点序列分型 |
Molecular typing and drug resistance analysis of Salmonella typhi in Xiantao City from 2014 to 2018 |
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| YE Hengping,ZHANG Yating,YUAN Min,XING Xuesen,LYU Jing,HE Fei,ZHOU Haijian,LI Guoming,YANG Hongmei. Molecular typing and drug resistance analysis of Salmonella typhi in Xiantao City from 2014 to 2018[J]. International Journal of Laboratory Medicine, 2020, 0(9): 1042-1046,1051 |
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| Authors: | YE Hengping ZHANG Yating YUAN Min XING Xuesen LYU Jing HE Fei ZHOU Haijian LI Guoming YANG Hongmei |
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| Affiliation: | (Xiantao Center for Disease Control and Prevention,Xiantao,Hubei 433000,China;Hubei Provincial Center for Disease Control and Prevention,Wuhan,Hubei 430079,China;Chinese Center for Disease Control and Prevention,Beijing 100026,China) |
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| Abstract: | Objective To understand the drug resistance and molecular analysis of Salmonella typhi in Xiantao City.Methods Totally 29 strains of Salmonella typhi isolated from 2014 to 2018 were tested for drug sensitivity,pulsed field gel electrophoresis(PFGE)typing and multiple locus sequence typing(MLST).Results The results of drug sensitivity showed that 27 of 29 Salmonella typhi strains were sensitive/moderately sensitive to 26 kinds of common clinical antibiotics,and the other 2 strains were resistant to different degrees,one of which was widely distributed and resistant toβ-lactam/β-lactam inhibitor complex.The results of PFGE showed that 29 strains of Salmonella typhi could be divided into 6 types of PFGE,among which the dominant type was HuBXTSal003.MLST showed that 29 strains had two sequence types,ST2 was the dominant ST type.Conclusion There are dominant PFGE and ST types of Salmonella typhi in Xiantao City,and there are multi drug resistant strains. |
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| Keywords: | Salmonella typhi drug sensitivity test pulsed field gel electrophoresis multilocus sequence typing |
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