红景天苷通过miRNA-210-3p/E2F3抑制非小细胞肺癌细胞的增殖和迁移

目的:探讨红景天苷（salidroside，Sal）通过miRNA-210-3p/E2F3对非小细胞肺癌（non-small cell lung cancer，NSCLC）细胞增殖和迁移的影响及作用机制。方法:通过生物信息网站分析miR-210-3p及其靶基因在肺腺癌组织和正常组织中的表达及生存影响。采用免疫组化法检测肺腺癌组织和正常组织中E2F3蛋白的表达；qRT-PCR检测NSCLC细胞中miR-210-3p和E2F3基因表达；Western blot检测NSCLC细胞中E2F3蛋白表达；CCK-8、细胞划痕及克隆形成实验分别检测红景天苷和miR-210-3p对NSCLC细胞增殖、迁移及克隆形成的影响。结果:生物信息网站显示miR-210-3p和E2F3在肺腺癌组织中高表达，且与不良预后相关。Sal以时间-浓度依赖抑制NSCLC细胞的增殖活性（P＜0.05），并显著降低NSCLC细胞的迁移及克隆形成能力（P＜0.05），而低浓度Sal对正常肺上皮细胞的增殖无明显抑制效果。miRNA-210-3p和E2F3表达在NSCLC细胞中上调（P＜0.05），Sal可抑制二者表达（P＜0.05）。与对照组比较，miR-210-3p inhibitor组E2F3表达上调（P＜0.05），miR-210-3p mimics组E2F3表达被抑制（P＜0.05），Sal与miR-210-3p mimics联用进一步抑制了miR-210-3p mimics所致的E2F3表达下调和细胞增殖和迁移的增强作用（P＜0.05）。结论:miR-210-3p和E2F3在肺腺癌中高表达，Sal可通过时间-浓度依赖负调控miRNA-210-3p/E2F3影响 NSCLC细胞的生物学行为，发挥抗癌作用。

Salidroside suppresses proliferation and migration of non-small cell lung cancer cells via miRNA-210-3p/E2F3

Objective:To investigate the effect and mechanism of salidroside (Sal) on proliferation and migration of non-small cell lung cancer (NSCLC) cells through miRNA-210-3p/E2F3.Methods:The expression and survival effects of miR-210-3p and target gene in lung adenocarcinoma tissues and normal tissues were analyzed by bioinformatics sites.Immunohistochemistry was used to detect the expression of E2F3 protein in lung adenocarcinoma tissues and normal tissues.qRT-PCR was used to detect the expression of miR-210-3p and E2F3 in NSCLC cells.Western blot was used to detect the expression of E2F3 protein in NSCLC cells.The effects of Sal and miR-210-3p on proliferation,migration and colony formation of NSCLC cells were determined by CCK-8 assay,scratch assay and colony formation assay,respectively.Results:Bioinformatics site showed that miR-210-3p and E2F3 were highly expressed in lung adenocarcinoma tissues and were associated with poor prognosis.Sal inhibited the proliferation activity of NSCLC cells in a time-concentration dependent manner (P＜0.05),and significantly decreased the migration and colony formation ability of NSCLC cells (P＜0.05),while low concentrations of Sal had no significant inhibitory effect on the proliferation of normal lung epithelial cells.miRNA-210-3p expression and E2F3 expression were upregulated in NSCLC cells (P＜0.05),but Sal could inhibit their expression (P＜0.05).Compared with the control group,the expression of E2F3 was increased in the miR-210-3p inhibitor group (P＜0.05),inhibited in the miR-210-3p mimics group (P＜0.05).The combination of Sal and miR-210-3p mimics further inhibited the downregulation of E2F3 expression induced by miR-210-3p mimics and the enhancement of NSCLC cell proliferation and migration (P＜0.05).Conclusion:miR-210-3p and E2F3 were highly expressed in lung adenocarcinoma,and Sal could affect the biological behavior of NSCLC cells and exert anticancer effects via negative regulation of miRNA-210-3p/E2F3 in a time-concentration dependent manner.