子宫内膜癌组织中HAX1和突变型p53表达的相关性及其临床意义

目的:探讨造血干细胞特异性相关结合蛋白1（HAX1）和突变型p53在子宫内膜癌组织中的表达模式、相关性及与预后的关系。方法:采用UALCAN数据库及qRT-PCR分析新鲜子宫内膜癌组织中HAX1 mRNA的表达水平；利用Western blotting检测新鲜子宫内膜癌组织中HAX1的蛋白表达水平；应用免疫组化检测120例子宫内膜癌患者癌组织及相应的癌旁组织石蜡标本中HAX1和p53蛋白的表达水平，分析两者的相关性及与患者临床病理特征和预后的关系；利用GEPIA数据库对HAX1与子宫内膜癌相关基因的关系进行分析。结果:HAX1 mRNA和蛋白在子宫内膜癌组织中均高表达；HAX1阳性表达和突变型p53的表达中度正相关（χ2=36.468，r=0.551，P<0.05），且均与淋巴结转移、FIGO分期、血CEA水平以及Ki-67的表达密切正相关（P<0.05），但与患者总生存率呈负相关；HAX1阳性表达（P=0.008）、突变型p53表达（P=0.022）、淋巴结有转移（P=0.003）、FIGO高分期（P<0.001）及术后辅助治疗方法（P<0.001）是患者预后的独立危险因子；基因相关性分析结果显示HAX1与TP53、MKI67、BAX、CCNE1、POLE1、MSH2、MSN6及PMS2的表达相关。结论:HAX1在子宫内膜癌组织中高表达，与突变型p53表达正相关，两者表达模式可综合评估该肿瘤的恶性程度、进展情况及患者的预后情况，为子宫内膜癌的术后治疗提供新思路。

Correlation and clinical value of HAX1 and mutant-p53 expression in endometrial carcinoma tissue

Objective:To study the expression levels of hematopoietic stem cell specific associated binding protein-1(HAX1) and mutant-p53 expression in endometrial carcinoma(EC),and the correlation with prognosis of EC.Methods:UALCAN database and qRT-PCR were used to check the expression levels of HAX1 mRNA in fresh EC tissues.Western blotting was used to check the expression levels of HAX1 protein in fresh EC tissues.Immunohistochemical(IHC) method was used to detect the proteins levels of HAX1 and p53 in a total of 120 paraffin EC and adjacent normal tissues and their correlation.The relationships between HAX1 and p53 and clinicopathological features of EC and the prognosis of patients were further analyzed.GEPIA database was used to further analyze the correlation between HAX1 and EC related genes.Results:HAX1 mRNA and protain were highly expressed in EC.The protein expressions of HAX1 and mutant-p53 were significantly positively correlated(χ2=36.468，r=0.551，P<0.05),and both were closely related to lymph node metastasis,FIGO staging,blood CEA level and Ki-67 expression(P<0.05),but negatively correlated with overall survival of patients with EC.HAX1 positive expression（P=0.008）,p53 mutant expression（P=0.022）,lymph node metastasis（P=0.003）,FIGO high stage（P<0.001）and postoperative adjuvant therapy（P<0.001） were independent risk factors for prognosis of EC patients.Also,HAX1 was correlated with the expression of TP53,MKI67,BAX,CCNE1,POLE1,MSH2,MSN6 and PMS2.Conclusion:HAX1 is highly expressed in EC,and positively correlated with mutant-p53 expression.The expression mode of the two could comprehensively evaluate the malignancy,progression of the cancer and prognosis of the patients,providing new ideas for the postoperative treatment of EC.