SGPL1在神经胶质瘤组织中的表达及其对细胞增殖和凋亡的影响

目的:探讨SGPL1在神经胶质瘤组织中的表达及其对神经胶质瘤细胞增殖和凋亡的影响。方法:在TCGA及PROGgene在线数据库中分析SGPL1在神经胶质瘤临床肿瘤组织及正常神经组织中的表达差异，并分析其表达与神经胶质瘤预后生存的关系；在神经胶质瘤细胞系U251和U87中分别敲降和过表达SGPL1，通过RT-qPCR和Western blot实验分别检测SGPL1的mRNA和蛋白表达水平；用CCK-8法、细胞克隆形成实验检测SGPL1敲降和过表达后细胞增殖变化；通过流式细胞术和caspase3/7酶活性试剂检测分析细胞凋亡变化，Western blot实验检测caspase3、PARP及CyclinD1等凋亡相关蛋白的表达；ELISA检测细胞内S1P含量；RT-qPCR检测S1PR5的表达水平;RNA-seq分析SGPL1在神经胶质瘤细胞中调控的信号通路；Western blot实验检测SGPL1敲降和过表达后Phospho-p38-MAPK、Phospho-ERK、Phospho-STAT3及Phospho-NF-κB(p65)的表达变化。结果:TCGA及PROGgene在线数据库分析表明SGPL1在神经胶质瘤组织中高表达并与神经胶质瘤患者的预后生存成负相关。在U251中敲降SGPL1后细胞增殖受到抑制，细胞凋亡增加；在U87细胞中过表达SGPL1后能显著促进细胞的增殖能力。SGPL1表达对S1P信号通路无明显影响；RNA-seq结果表明SGPL1在神经胶质瘤细胞内调控细胞增殖死亡信号通路，SGPL1敲降和过表达可影响p38-MAPK、ERK、STAT3及NF-κB(p65)等细胞增殖生长信号的变化。结论:SGPL1在神经胶质瘤组织中高表达并与生存预后负相关。在神经胶质瘤细胞内SGPL1可调控p38-MAPK、ERK、STAT3及NF-κB(p65)等细胞增殖生长信号通路，其表达可影响神经胶质瘤细胞的增殖和凋亡，可能是一个潜在的肿瘤标志物和治疗的分子靶点。

The expression of SGPL1 in glioma tissue and its effect on the proliferation and apoptosis of glioma cells

Objective:To investigate the expression of SGPL1 in glioma tissue and the effect of SGPL1 expression on the proliferation and apoptosis of glioma cells.Methods:The differential expression of SGPL1 in glioma clinical tumor tissue and normal nerve tissue was analyzed by the TCGA and PROGgene online database,and the relationship between its expression and prognostic survival in glioma patients was analyzed.Knockdown and overexpression of SGPL1 were performed in the glioma cell lines U251 and U87,respectively.The mRNA and protein expression levels of SGPL1 were determined by the RT-qPCR and Western blot.After SGPL1 knockdown and overexpression,cell proliferation was detected by CCK-8 assay and cell cloning assay.The apoptosis was analyzed by flow cytometry and caspase3/7 enzyme activity reagent.The expressions of caspase3,PARP and CyclinD1 were detected by Western blot.The content of S1P in the cells was detected by ELISA.RT-qPCR was used to detect the expression of S1PR5.RNA-seq was used to analyze the signaling pathway regulated by SGPL1 in glioma cells.Western blot assay was used to detect Phospho-p38-MAPK,Phospho-ERK,Phospho-STAT3,and Phospho-NF-κB(p65) expression after SGPL1 knockdown and overexpression.Results:TCGA and PROGgene online database analysis showed that SGPL1 was highly expressed in glioma tissues and negatively correlated with the prognostic survival of glioma patients.After SGPL1 knockdown in U251 cells,cell proliferation was inhibited and apoptosis was increased.Overexpression of SGPL1 in U87 cells significantly promoted cell proliferation.The expression of SGPL1 had no significant effect on S1P signaling pathway.RNA-seq results showed that SGPL1 regulated the cell proliferation and death signaling pathway in glioma cells.Knockdown and overexpression of SGPL1 affected the proliferation and growth signaling pathways of p38-MAPK,ERK,STAT3 and NF-κB(p65).Conclusion:SGPL1 is highly expressed in gliomas tissue and negatively correlated with survival prognosis.SGPL1 regulates the cell proliferation and growth signaling pathways of p38-MAPK,ERK,STAT3 and NF-κB(p65) in glioma cells,and its expression can affect the proliferation and apoptosis of glioma cells,which may be a potential tumor marker and therapeutic molecular target.