circRNA＿0016418及circRNA＿0017247异常表达对恶性黑色素瘤诊断及其预测淋巴结转移的价值

目的:探讨circRNA＿0016418及circRNA＿0017247异常表达对恶性黑色素瘤诊断及其预测淋巴结转移的价值。方法:选取2018年01月至2021年10月我院收治的68例恶性黑色素瘤患者，将其分为淋巴结转移组26例和无淋巴结转移组42例。采用实时荧光定量PCR法检测恶性黑色素瘤组织及正常组织中circRNA＿0016418及circRNA＿0017247的水平。应用受试者工作特征(receiver operating characteristic, ROC)曲线分析circRNA＿0016418及circRNA＿0017247异常表达对恶性黑色素瘤诊断及其预测淋巴结转移的价值。Pearson相关分析检测恶性黑色素瘤患者中circRNA＿0016418水平与circRNA＿0017247水平的相关性。结果:恶性黑色素瘤组织中circRNA＿0016418水平(2.64±1.17 vs 0.95±0.36)及circRNA＿0017247水平(5.18±2.50 vs 1.52±0.63)均明显高于正常组织(P<0.001)。淋巴结转移组circRNA＿0016418水...

The value of abnormal expression of circRNA_0016418 and circRNA_0017247 in diagnosis of malignant melanoma and prediction of lymph node metastasis

Objective:To investigate the value of abnormal expression of circRNA_0016418 and circRNA_0017247 in the diagnosis of malignant melanoma and the prediction of lymph node metastasis.Methods:The 68 patients with malignant melanoma in our hospital from January 2018 to October 2021 were selected and divided into lymph node metastasis group (n=26) and non-lymph node metastasis group (n=42).The circRNA_0016418 and circRNA_0017247 levels in malignant melanoma tissues and normal tissues were detected by real-time fluorescence quantitative PCR.The receiver operating characteristic (ROC) curve was used to analyze the value of abnormal expression of circRNA_0016418 and circRNA_0017247 in the diagnosis of malignant melanoma and its prediction of lymph node metastasis.Pearson correlation analysis detected the correlation between circRNA_0016418 level and circRNA_0017247 level in patients with malignant melanoma.Results:The levels of circRNA_0016418 (2.64±1.17 vs 0.95±0.36) and the levels of circRNA_0017247 (5.18±2.50 vs 1.52±0.63) in malignant melanoma tissues were significantly higher than those in normal tissues (P＜0.001).The levels of circRNA_0016418 (3.85±1.72 vs 1.66±0.74) and the levels of circRNA_0017247 (7.16±3.82 vs 3.38±1.54) in the group with lymph node metastasis were significantly higher than those in the group without lymph node metastasis (P＜0.001).ROC curve showed that the area under the curve of circRNA_0016418 and circRNA_0017247 for the combined diagnosis of malignant melanoma was 0.905 (95%CI:0.847~0.964),with sensitivity of 92.8% and specificity of 83.5%.The area under the curve of circRNA_0016418 combined with circRNA_0017247 to predict lymph node metastasis was 0.957 (95%CI:0.895~0.993),with sensitivity of 98.0% and specificity of 84.6%.Correlation analysis showed that circRNA_0016418 level was positively correlated with circRNA_0017247 level in patients with malignant melanoma (r=0.802,P＜0.001).Conclusion:The levels of circRNA_0016418 and circRNA_0017247 in malignant melanoma tissues are significantly increased,which are associated with lymph node metastasis.The combined detection of the two is of great value in the diagnosis of malignant melanoma and the prediction of lymph node metastasis.